Gas Chromatography-Mass Spectrometry (GC-MS)

Purpose:

Qualitative and quantitative determination of the monosaccharide composition of glycoconjugate, oligosaccharide, or polysaccharide samples.

Determination of the linkage positions of sugars in an oligosaccharide or a polysaccharide.

Method used:

Gas chromatography-mass spectrometry (GC-MS) is usually used for the carbohydrate composition of samples originating from plants or microbes.



 This analysis tests for the following carbohydrates:
arabinose                            rhamnose
fucose                                 xylose
glucuronic acid                    galacturonic acid
mannose                             galactose
glucose                               N-acetyl-mannosamine
N-acetyl-galactosamine      N-acetyl-glucosamine



• Gas chromatography-mass spectrometry (GC-MS) is also used for the determination of the linkage positions of sugar in an oligosaccharide or a polysaccharide.

      This analysis test for the linkage positions of the following carbohydrates:
arabinose                            rhamnose
fucose                                 xylose
glucuronic acid                    galacturonic acid
mannose                             galactose
glucose