Glycosyl Residue Composition Analysis of Recombinant and Native Glycoproteins

Purpose: 
Quantitative determination of glycosyl residue and glycosyl-linkage composition analysis in glycoproteins.

Methods Used:        
Glycosyl residue composition of glycoproteins is usually done by HPAEC because it has the capacity to detect monosaccharide from a very limited amount of sample.  In addition, HPAEC is the method of choice for the determination of sialic acids.  The monosaccharide present in glycoproteins can be analyzed also by GC-MS; however, the sample submitted should provide 2 to 8 µg per sugar residue (at least 500 µg) to obtain a reliable composition data.  For detailed methods of glycosyl residue composition, click on the following links: Monosaccharide Composition Analysis by HPAEC-PAD, and Glycosyl-Residue Composition Analysis by GC-MS.