The Complex Carbohydrate Research Center (CCRC) of The University of Georgia offers a comprehensive service program for structurally characterizing glycoconjugates from animal, bacterial, fungal, and plant sources. We also offer courses on carbohydrate analysis on a yearly basis. Please see our training page for information on upcoming courses on carbohydrate analysis.

The available analyses and techniques include:

Routine Analyses

• Glycosyl composition analysis by GC-MS and HPAEC
• Glycosyl linkage analysis by GC-MS
• Molecular weight determination of oligosaccharides and glycopeptides
• Molecular weight determination and purification of polysaccharides

Recombinant and Native Glycoprotein Analyses

• Glycosyl-residue compositions in oligosaccharide(s) and glycoprotein
• Glycosyl-linkage analysis in oligosaccharides
• Identifying site(s) of attachment of N- and O-linked glycans
• Purification and oligosccharides analysis of N- and O-linked glycans
• Relative quantitation of N- and O-linked glycans by isotopic permethylation using 13CH3I
• Determining glycosyl residue sequence, ring size, and anomeric configuration
• Identifying and determining the points of attachment of non-carbohydrate constituents such as phosphate and sulfate

Transcript analysis of glycan related genes by qRT-PCR

• We have developed a high-throughput methodology for transcript analysis of glycan related genes (transcripts from > 700 genes) by a qRT-PCR approach. The power of the technique is its extreme sensitivity and the side dynamic range for quantitation of both high and low abundance transcripts. The focus of our studies will be to assess transcript abundance by qRT-PCR for genes encoding all of the “glycans-related” glycosyltransferases, glycosidases, transporters, modification enzymes, sugar-nucleotide biosynthetic enzymes and lectins (>700 genes). Interested parties should feel free to contact Dr. Kelley Moremen or Dr. Parastoo Azadi.
• Click here for Glycan Biosynthetic Pathways and "Glycotranscriptome" Analysis site

Extracellular Polysaccharides, Lipopolysaccharides and Glycolipids

• Purification
• Composition Analysis
• Linkage Analysis
• Identification of Lipid Anchors

New Services

• Services Being Offered for Glycosaminoglycan (GAG) Structural analysis
• Enzyme digestion of glycosaminoglycans to disaccharides
  
• Identification of disaccharides and sulfation pattern by online ESI-MS
  and ESI-MS/MS
• Identification of released disaccharides by Strong -Anion-Exchange
  Chromatography and detection of the disaccharides by both UV and
  fluorescent detection
• Structural characterization of GAG oligosaccharides by 1D and 2D NMR
• Sequencing of GAG oligosaccharides by ESI-MS and ESI-MS/MS
• Glycosyl composition analysis of GAGs by GC-MS
  

Mass Spectrometry and Liquid Chromatography

• Matrix-Assisted Laser Desorption Mass Spectrometry (MALDI-MS)
• Electrospray Ionization Ion Trap Mass Spectrometry (ESI-MS/MS)
• High pH Anion Exchange Chromatography (HPAEC)
• Gas Chromatography (GC) and Gas Chromatography-Mass Spectrometry (GC-MS)

Nuclear Magnetic Resonance (NMR) Instruments

• 300 MHz (2 channel, gradients)
• 500 MHz (89mm wide-bore manget for solids and liquids, 3 channel, gradients)
• 600 MHz (3 channel, gradients)
• 800 MHz (3 channel, gradients)

We offer routine 1D 1H NMR and 13C NMR spectroscopy as well as 2D NMR experiments such as COSY, TOCSY, HSQC.

Custom Chemical Synthesis

• Synthesis of oligosaccharides and glycoconjugates
• Synthesis of glycopeptides
• Use a combination of chemical and enzymatic approaches

Molecular Modeling

• Generate input files for running AMBER MD
• Build and view carbohydrate structures
• Generate the coordinate and topology files for carbohydrate and protein simulations

Please click here to fill out a request for analytical services

CCRC will not start sample analysis until a request form has been completed and submitted.
For more information about our services, please contact Dr. Parastoo Azadi.
Dr. Parastoo Azadi, Technical Director
Complex Carbohydrate Research Center
315 Riverbend Rd., Athens, GA 30602-4712
Telephone:  706-583-0629
FAX:  706-542-4412
azadi@ccrc.uga.edu


Information about potential collaborations can be obtained by contacting Dr. Azadi or the appropriate faculty or staff member listed on our web site: www.ccrc.uga.edu