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ANALYTICAL SERVICES

The Complex Carbohydrate Research Center (CCRC) of The University of Georgia offers a comprehensive service program for structurally characterizing glycoconjugates from animal, bacterial, fungal, and plant sources. We also offer courses on carbohydrate analysis on a yearly basis. Please see our training page for information on upcoming courses on carbohydrate analysis.

The available analyses and techniques include:

Routine Analyses:

    · Glycosyl composition analysis by GC-MS and HPAEC
    · Glycosyl linkage analysis by GC-MS
    · Molecular weight determination of oligosaccharides and glycopeptides

Recombinant and Native Glycoprotein Analyses:

    · Glycosyl-residue and glycosyl-linkage compositions
    · Identifying site(s) of attachment of N- and O-linked glycans
    · Releasing and purifying N- and O-linked glycans
    · Identifying the type of N-linked glycans, e.g. biantennary, triantennary, tetraantennary, high mannose, hybrid, complex
    · Determining glycosyl residue sequence, ring size, and anomeric configuration
    · Identifying and determining the points of attachment of non-carbohydrate constituents such as phosphate and sulfate

Polysaccharide, Lipopolysaccharide, Glycosphingolipid, and Glycophosphoinositide Analyses:

    · Purifying to homogeneity and determining glycosyl-residue and glycosyl-linkage compositions
    · Sequencing oligosaccharide or polysaccharide repeating units including identity, linkage, ring size, and anomeric configuration of each
      glycosyl residue
    · Detecting and, when possible, identifying the presence of unusual glycosyl residues
    · Identifying non-carbohydrate constituents such as acetyl or pyruval groups
    · Identifying and analyzing the lipid moiety, e.g. lipid A, ceramide, or diacylglycerol

Mass Spectrometry and Liquid Chromatography:

    · Matrix-Assisted Laser Desorption Mass Spectrometry (MALDI-MS)
    · Quadrupole/ Time-of-Flight Mass Spectrometry (Q-TOF) ESI-MS/MS
    · Ion trap electrospray lonization Mass Spectrometry (ESI-MS/MS)
    · High pH Anion Exchange Chromatography (HPAEC)
    · Gas Chromatography (GC) and Gas Chromatography-Mass Spectrometry (GC-MS)

Nuclear Magnetic Resonance (NMR) Instruments:

    · 300 MHz (2 channel, gradients)
    · 500 MHz (89mm wide-bore magnet for solids and liquids, 3channel, gradients)
    · 600 MHz (3 channel, gradients)
    · 800 MHz (3 channel, gradients)

We offer routine 1D 1H NMR and 13C NMR spectroscopy as well as 2D NMR experiments such as COSY, TOCSY, HSQC.

Custom Chemical Synthesis

    · Synthesis of oligosaccharides and glycoconjugates
    · Synthesis of glycopeptides
    · Use a combination of chemical and enzymatic approaches
 

Molecular Modeling

    The AMBER/GLYCAM web interface tool (http://glycam.ccrc.uga.edu)
    · Generate input files for running AMBER MD
    · Build and view carbohydrate structures
    · Generate the coordinate and topology files for carbohydrate and protein simulations
 
 

Transcript analysis of glycan related genes by qRT-PCR.

We have developed a high-throughput methodology for transcript analysis of glycan related genes (transcripts from >700 genes) by a qRT-PCR approach. The power of the technique is its extreme sensitivity and the wide dynamic range for quantitation of both high and low abundance transcripts. The focus of our studies will be to assess transcript abundance by qRT-PCR for genes encoding all of the "glycan-related" glycosyltransferases, glycosidases, transporters, modification enymes, sudar-nucleotide biosynthetic enzymes and lectins (>700 genes). Interested parties should feel free to contact Dr. Kelley Moremen or Dr. Parastoo Azadi

Relative quantitation of N and O-linked glycans by isotopic permethylation using 13CH3I and Mass spectrometry.

Click here for Glycan Biosynthetic Pathways and "Glycotranscriptome" Analysis site

Please click here to fill out a request for analytical services . CCRC will not start sample analysis until a request form has been completed and submitted.

For more information about our services, please contact Dr. Parastoo Azadi.