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We have developed a high-throughput methodology for transcript analysis of glycan related genes (transcripts from >700 genes) by a qRT-PCR approach. The power of the technique is its extreme sensitivity and the wide dynamic range for quantitation of both high and low abundance transcripts. The focus of our studies will be to assess transcript abundance by qRT-PCR for genes encoding all of the "glycan-related" glycosyltransferases, glycosidases, transporters, modification enymes, sudar-nucleotide biosynthetic enzymes and lectins (>700 genes). Interested parties should feel free to contact Dr. Kelley Moremen or Dr. Parastoo Azadi (azadi@ccrc.uga.edu ).