Glycosyl composition analysis by GC-MS and HPAEC
Glycosyl composition analysis by GC-MS and HPAEC
Purpose:
Qualitative and quantitative determination
of the monosaccharide composition of glycoconjugate, oligosaccharide, or
polysaccharide samples.
Methods Used:
Gas chromatography-mass spectrometry (GC-MS)
or high-pH anion-exchange chromatography (HPAEC)
is used for composition analysis.
Amount of Sample
Required for Analysis:
If you have no knowledge of the nature
of the sugar constituents of your sample and you desire a complete composition
analysis, a minimum of 100 micrograms of carbohydrate is required. If you
have considerably smaller amounts of carbohydrate sample, we can make special
arrangements to determine the composition of your sample. Please contact
us to discuss your specific requirements, and do not let limited sample
amount deter you from requesting our services.
General Criteria
for Sample Preparation:
Samples must be free of salts. If the
sample is a glycoprotein, it can be dialyzed and then lyophilized. If the
sample is an oligosaccharide, it can be desalted by size-exclusion chromatography
on a Bio-Gel P-2 or Sephadex G-10 column.
Please click here to fill out a request for analytical services.
