Glycosyl Linkage Analysis
Glycosyl Linkage Analysis
Purpose
Determination of the linkage positions
of sugars in an oligosaccharide.
Methods Used
Methylation analysis, gas chromatography-mass
spectrometry (GC-MS).
Amount of Sample
Required for Analysis
A minimum of 100-200 micrograms of carbohydrate
is required, more if available.
General Criteria
for Sample Preparation
Your sample must be extremely pure, as
a mixture will not yield much useful information. Glycosyl composition
data are usually required prior to glycosyl linkage (methylation) analysis.
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