Electrospray Ionization-Mass Spectrometry (ESI-MS)
Purpose
Determination of
the molecular weight of oligosaccharides, glycopeptides, glycoproteins,
and proteins.
Amount
of Sample Required for Analysis
A minimum of 200
picomoles of carbohydrate is required.
General Criteria for Sample Preparation
Your sample should
be pure, soluble in water or 50% methanol, and free of non-volatile salts,
buffers, and detergents. ESI-MS can be performed on samples up to 100 kDa.
ESI-MS produces a series of multiply charged ions, as compared with [M+H]+
in other mass spectrometric procedures.
On-Line Liquid Chromatography-Electrospray Ionization-Mass Spectrometry (LC-ESI-MS)
Purpose
On-line separation
and determination of the molecular weight of peptides and glycopeptides.
Amount
of Sample Required for Analysis
A minimum of 400
picomoles of peptides or glycopeptides is required.
For specialized mass spectrometric analyses/collaborations:
Dr.
Ron Orlando,
Head,
Mass Spectrometry Laboratory
orlando@ccrc.uga.edu,
706-542-4429
Please
click here to fill out a request for analytical services.