Orlando
MS Lab
Complex Cabohydrate Research
Center
University of Georgia
View our Instrumentation:
View our Group Members and their
Work
Visit the AAMSDG: Atlanta-Athens
Mass Spectrometry Discussion Group
Visit the CCRC
or UGA:
Useful websites for MS and biological
analysis
Search
the CCRC database for publications from our group.
Jeremi
Johnson: My research incorporates a multi-disciplinary foundation
involving the combination of analytical chemistry and biochemistry.
I work on characterizing post-translational modifications (glycosylation
and phosphorylation) present on proteins, detection and characterization
of anitbody specific proteins, and development of new methods for high
throughput protein analysis (qualitative and quantitative). Analytical
techniques and bioanalytical methods utilized include mass spectrometry
(MALDI, ESI, nanospray, LC-MS, LC-MSMS), RP-HPLC, hydrophilic interaction
chromatography (HILIC), 1-D and 2-D gel electrophoresis, protein electro-blotting,
enzyme-linked immuno-sorbent assays (ELISA), in-gel and solution phase
protein digestions, and several others.
Min
Xie: My research interest focuses on two closely related
aspects of portein analysis by mass spectrometry. One is the development
of carbohydrate, glycopeptide, glycoprotein characterization assay. The
structural complexity, thermal lability and nonvolatility of those biomolecules
keep them a challenge for mass spectrometry analysis. My work combines
site-spectified chemical/enzyme digestions, affinity separation via antibody-antigen
recongization mechanism and chemical lableing methods to decrease sample's
complexity, increase the purification so that improve the MS sensitivity.
Another work is the characterization of protein post-translational modifications
which includes glycosylation and phosphorylation. The techniques applied
in my work include various Mass Spectrometry (MALDI, ESI, Nanospray and
MS/MS); chromatography (RP-HPLC, affinity LC, ion-exchange, size-exclusion);
LC-MS (either online or offline) and LC-MS/MS; 1-D PAGE-SDS and 2-D gel
electrophoresis as well as ELISA, lectin-blotting techniques etc.
Haofei
Wang:
I am interested in bioinformatics/biostatistics methods on proteomics
study. My research has focused on protein identification using Mass Spectrometry
technique (MALDI-TOF) combining with Database Searching utilities. The
success of database mining appears to be dependent upon protein purity,
peptide yield, genomic complexity of target organism and accuracy of peptide
mass assignment. My work investigates the dynamic interaction of these
variables and their effect on database mining.
For more about me, please visit
my webpage
Tasneem
Bahrainwala: I will be focusing my research time on the development
of hydrogen/deuterium exchange - MS and MS/MS procedure for the characterization
of protein-carbohydrate and protein-protein binding interactions.
James
Atwood: My research involves the selective separation of
proteins based on their isoelectric point utilizing cation exchange chromatography.
In addition future work will involve 2D chromatography utilizing cation
exchange and reverse phase chromatographies. This combination will
be utilized to help fractionate extremely complex protein mixtures such
that LC-MS can be utilized more effectively for the characterization low
number proteins or protein mixtures.
Useful
Websites
Protein Prospector
Prowl
Matix Science
OCA pdb query
NIH pdb query
CCRC, UGA,
220 Riverbend Rd,
Athens, GA 30602
phone: 706-542-4401
fax: 706-542-4412