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Kelley Moremen
Distinguished Research Professor of Biochemistry and Molecular Biology


E-mail: moremen@uga.edu
Telephone: 706-542-1705
Fax: 706-542-4412

Short Biography
Research Interests
Publications
Lab-personal web site

Short Biography:
Dr. Moremen received his B.S. in Biology and Chemistry in 1978 from Dickinson College and his Ph.D. in Molecular Biology in 1984 from Vanderbilt University. Prior to joining the faculty at the CCRC in 1991, he spent five years as a Postdoctoral Fellow at Massachusetts Institute of Technology. In 2013, Dr. Moremen was elected chair of the 2013 Glycobiology Gordon Research Conference, served on the Board of Directors of the Society for Glycobiology, as well serving since 2005 as Secretary of the Society. He has served on editorial boards of Journal of Biological Chemistry, Glycobiology, and Glycoconjugate Journal, numerous NIH grant review panels, and Scientific Advisory Boards of four biotech companies. In 2014 Dr. Moremen was appointed the Distinguished Research Professorship in Biochemistry and Molecular Biology at the University of Georgia. Dr. Moremen has 9 patents and over 100 publications.

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Research Interests:
Research in the Moremen lab focuses on the biochemistry, structure, and regulation of enzymes involved in the biosynthesis, recognition, and catabolism of mammalian glycoproteins. Carbohydrate structures on glycoproteins contribute to many biological recognition events during development, oncogenic transformation, and cell adhesion. Large numbers of intracellular and extracellular proteins contain covalently bound glycans and alterations in the synthesis and degradation of these structures can occur in human genetic diseases and cancer. Many questions remain regarding the regulation of glycosylation pathways, the structures and functions of the processing enzymes, and the specificity and regulation of protein-carbohydrate interactions during development. Research in the Moremen lab is focused on developing novel strategies to address contemporary problems in the regulation, roles, mechanism, and machinery of mammalian protein glycosylation with efforts in four main areas:

1) Structures, specificities and mechanisms of mammalian glycosylation enzymes: Studies on the mammalian glycosylation enzymes in the Moremen lab have expanded in recent years with the development of a unified design and expression platform (Repository of Glycan-related Expression Constructs) for recombinant production of all mammalian glycosyltransferases, glycosidases, and sulfotransferases (target gene list of >300 coding regions) in bacteria, baculovirus, and mammalian cells. This multi-investigator effort, initiated and coordinated within the Moremen lab, is focused on production of these recombinant enzymes and has led to new enzyme structures and insights into the structural basis for enzymatic glycan synthesis. Expansion of this project is leading to additional applications in chemoenzymatic synthesis, biochemical and structural studies, and development of technologies for enzymatic modification of glycoproteins and glycolipids in cellular environments to monitor molecule redistribution in the course of disease. The goals of these studies are to provide a biochemical and structural understanding of glycan biosynthesis and catabolism as well as providing enzymatic catalysts for chemoenzymatic synthesis. This project is funded by an NIH P01 grant (P01GM107012, Mammalian Glycosyltransferases for Use in Chemistry and Biology). For further information please see: glycoenzymes.ccrc.uga.edu.

2) Protein-carbohydrate interactions: Cell-surface and extracellular glycoproteins and glycolipids play unique and critical roles in mammalian physiology. One of the major glycan classes at the cell surface and within the extracellular matrix are the proteoglycans (PGs) that play diverse roles as co-receptors for cell surface signaling, scaffolds for cell-matrix interactions, ligands that create morphogen or chemokine gradients in development and inflammation, and numerous other contributions to signaling and cell surface structure. Little is known about the details of PG interactions with binding partners or their mechanisms of biological function. The Moremen lab directs a multi-investigator project with goals to develop a novel, integrated technologies that will address the challenges of PG structures, interactions, and biological functions by leveraging advances in analytical, synthetic, structural, biochemical and biological tools. This project is funded by an NIH P41 grant (P41GM103390, Resource for Integrated Glycotechnology). For further information please see: glycotech.uga.edu.

3) Regulation of glycosylation machinery in mammalian systems: The Moremen lab is also involved in a collaborative project focused on determining the structures and regulation of glycans associated with glycoproteins and glycolipids in animal systems. The overall aims of the program are to examine the changes glycan structures during development with a particular focus on embryonic stem cell differentiation. The Moremen lab has developed platforms for analysis of glyco-gene transcript abundance using a custom real-time RT-PCR strategy as well as next-generation RNA-Seq methods. Integration of the transcript abundance data with the glycan structure analysis developed by other research groups in the CCRC will be accomplished through the bioinformatics group associated with the research program. This project is funded by an NIH P41 grant (P41GM103490, National Center for Biomedical Glycomics). For further information please see: glycomics.uga.edu

4) Structure and biochemistry of glycan trimming enzymes involved in glycoprotein biosynthesis and catabolism: The fourth area of focus in the Moremen lab is on the enzymes involved in glycan trimming in the secretory pathway that are essential for N-glycan maturation as well as playing a role in the catabolism of misfolded nascent glycoproteins in the endoplasmic reticulum (ER-associated degradation).

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Publications: Author's Last Name: Moremen

Journal Articles
Book Chapters are listed at the bottom of this page.

SM Halmo, D Singh, S. Patel, S Wang, M Edlin, GJ Boons, KW Moremen, D Live, L Wells. 2017. Protein O-linked mannose beta-1,4-N-acetyglucosaminyltransferase 2 (POMGNT2) is a gatekeeper enzyme for functional glycosylation of alpha-dystroglycan. J. Biol. Chem. 292(6): 2101-2109. PMID:27932460

MO Sheikh, SM Halmo, S Patel, D Middleton, H Takeuchi, CM Schafer, CM West, RS Haltiwanger, FY Avci, KW Moremen, L Wells. 2017. Rapid screening of sugar-nucleotide donor specificities of putative glycosyltransferases. Glycobiology 27: 206-212. PMID:28177478

AR Prudden, L Liu, CJ Capicciotti, MA Wolfert, S Wang, Z Gao, L Meng, KW Moremen, GJ Boons. 2017. Synthesis of asymmetrical multiantennary human milk oligosaccharides. Proc Natl Acad Sci USA 114(27): 6954-6959. PMID:28630345

S-H Yu, P Zhao, T Sun, Z Gao, KW Moremen, GJ Boons, L Wells, R Steet. 2016. Selective exo-enzymatic labeling detects increased cell surface sialoglycoprotein expression upon megakaryocytic differentiation. J. Biol. Chem. 291(8): 3982-3989. PMID:26733198

T Sun, Yu S-H, P Zhao, L Meng, KW Moremen, L Wells, R Steet, GJ Boons. 2016. One-step selective exoenzymatic labeling (SEEL) strategy for the biotinylation and identification of glycoproteins of living cells. J Am Chem Soc 138(36): 11575-11582. PMID:27541995

C. Zong, R. Huang, E. Condac, Y Chiu, W.Y. Xiao, X. Li, W Lu, M Ishahara, S. Wang, A. Ramiah, M Stickney, P. Azadi, IJ Amster, KW Moremen, L. Wang, JS Sharp, GJ Boons. 2016. Integrated approach to identify heparan sulfate ligand requirements of Robo1. J Am Chem Soc 138(39): 13059- 13067. PMID:27611601

Q Gao, C-Y Chen, CT Zong, S. Wang, A. Ramiah, P Prabhakar, L.C. Morris, GJ Boons, K. Moremen, J.H. Prestegard. 2016. Structural aspects of heparan sulfate binding to Robo1-lg1-2. ACS Chem Biol 11(11): 3106-3113. PMID:27653286

T Li, M Huang, L Liu, S Wang, KW Moremen, GJ Boons. 2016. Divergent chemoenzymatic synthesis of asymmetrical core fucosylated and core-unmodified N-glycans. Chem - Eur J 22(52): 18742-18746. PMID:27798819

JL Praissman, T Willer, MO Sheikh, A Toi, D Chitayat, YY Lin, H Lee, SH Stalnaker, S Wang, PK Prabhakar, SF Nelson, DL Stemple, SA Moore, KW Moremen, KP Campbell, L Wells. 2016. The functional O-manose glycan on alpha-dystroglycan contains a phospho-ribitol primed for matriglycan addition. eLife Apr 29:5: pii: e14473-doi:10.7554/eLif. PMID:27130732

SH Yu, P Zhao, T Sun, Z Gao, KW Moremen, GJ Boons, L Wells, R Steet. 2016. Selective Exo-Enzymatic Labeling Detects Increased Cell Surface Sialoglycoprotein Expression upon Megakaryocytic Differentiation. J Biol Chem 29(18): 3982-3989. PMID:26733198

Y Zhuo, JY Yang, KW Moremen, JH Prestegard. 2016. Glycosylation Alters Dimerization Properties of a Cell-surface Signaling Protein, Carcinoembryonic Antigen-related Cell Adhesion Molecule 1 (CEACAM1). J Biol Chem 291: 20085-20095. PMID:27471271

C Bello, S Wang, L Meng, KW Moremen, CFW Becker. 2015. A PEGylated Photocleavable Auxilary Mediates the Site-Specific Glycosylation and Native Chemical Ligation of Peptides. Angewandte Chemie In press.

Z. Liz, HA Moniz, A. Ramiah, KW Moremen, JS Sharp. 2015. High Structural Resolution Hydroxyl Radical Protein Footprinting Reveals an Extended Robo-1-Heparin Binding Interface. J. Biol. Chem. In press.

B. Zhang, W.Y. Xiao, H. Qiu, F. Zhang, HA Moniz, A Jaworski, E. Condac, G. Gutierrez-Sanchez, C. Heiss, RD Clugston, P. Azadi, JJ Greer, C. Bergmann, KW Moremen, D. Li, RJ Linhardt, JD Esko, L Wang. 2014. Heparan sulfate deficiency disrupts developmental angiogenesis and causes congenital diaphragmatic hernia. J. Clin. Invest. 124(1): 209-221. PMID:24355925

F Zhang, HA Moniz, B Walcott, KW Moremen, L Wang, RJ Linhardt. 2014. Probing the impact of GFP tagging on Robo1-heparin interaction.. Glycoconj. J. 31(4): 299-307. PMID:2014

JH Prestegard, DA Agard, KW Moremen, LA Lavery, LC Morris, K Pederson. 2014. Sparse labeling of proteins: structural characterization from long range constraints.. Magn Reson 241: 32-40. PMID:24656078

L Gasimli, AM Hickey, B Yang, G Li, M dela Rosa, AV Nairn, MJ Kulik, JS Dordick, KW Moremen, S Dalton, RJ Linhardt. 2014. Changes in glycosaminoglycan structure on differentiation of human embryonic stem cells towards mesoderm and endoderm lineages. Biochim Biophys Acta 1840(6): 1993-2003. PMID:24412195

JL Praissman, DH Live, S. Wang, A. Ramiah, ZS Chinoy, GJ Boons, KW Moremen, L. Wells. 2014. B4GAT1 is the priming enzyme for the LARGE-dependent functional glycosylation of alpha-dystroglycan.. eLife Oct 3;3: doi:10.7554/-eLife.03943. PMID:25279697

B.R. Urbanowicz, M.J. Pena, H.A. Moniz, K.W. Moremen, W.S. York. 2014. Two Arabidopsis proteins synthesize acetylated xylan in vitro. Plant J. 80: 197-206. PMID:25141999

C Zhang, Y Zhuo, HA Moniz, S Wang, KW Moremen, JH Prestegard, EM Brown, JJ Yang. 2014. Direct determination of multiple ligand interactions with the extracellular domain of the calcium-sensing receptor. J Biol Chem 289: 33529-33542. PMID:25305020

N.E. Mbua, X. Li, H.R. Flanagan-Steet, L. Meng, K. Aoki, K.W. Moremen, M.A. Wolfert, R. Steet, G.J. Boons. 2013. Selective exo-enzymatic labeling (SEEL) of N-glycans of living cells by recombinant ST6Gal. . Angewandte 52: 13012-13015. PMID:24129959

B. Zhang, W.Y. Xiao, H. Qiu, F.M. Zhang, H.A. Moniz, E. Condac, G. Gutierrez-Sanchez, C. Heiss, R.D. Clugston, P. Azadi, J.J. Greer, C. Bergmann, K.W. Moremen, D. Li, R.J. Linhardt, J.D. Esko, L. Wang. 2013. Congenital diaphragmatic hernia results from disruption of developmental angiogenesis mediated by Slit3-Robo4-heparan sulfate signaing. J. Clin. Invest. In press.

F.M. Zhang, H.A. Moniz, R.B. Walcott, K.W. Moremen, R.J. Linhardt, L. Wang. 2013. Characterization of the interaction between Robo1 and heparin and other glycosaminoglycans. Biochimie. 95(12): 2345-2353. PMID:23994753

L. Meng, F. Forouhar, D. Thieker, Z. Gao, A. Ramiah, H.A. Moniz, J. Seetharaman, S. Milaninia, M. Su, R. Bridger, L. Veillon, P. Azadi, G. Kornhaber, L. Wells, G.T. Montelione, R.J. Woods, L. Tong, K.W. Moremen. 2013. Enzymatic basis for N-glycan sialylation: Structure of mammalian alpha2,6-sialytransferase (ST6GAL1) reveals conserved and unique features for glycan sialytion. J. Biol. Chem. 288(48): 34680-34698. PMID:24155237

TA Gerken, L. Revoredo, JJC Thome, LA Tabak, MB Vester-Christensen, H. Clausen, GK Gahlay, DL Jarvis, RY Johnson, HA Moniz, KW Moremen. 2013. The lectin domain of the polypeptide GalNAc transferase family of glycosyltransferases (ppGalNAc Ts) acts as a switch directing glycopeptide substrate glycosylation in an N- or C-terminal direction, further controlling mucin type O-glycosylation. J. Biol. Chem. 288: 19900-19914. PMID:23689369

S Natunen, M Lampinen, H Suila, I Ritamo, V Pitkanen, AV Nairn, J Rabina, S Laitinen, KW Moremen, W Reutter, L Valmu. 2013. Metabolic glycoengineering of mesenchymal stromal cells with N-propanoylmannosamine. Glycobiology 23: 1004-1012. PMID:23708401

L Gasimli, HE Stansfield, AV Nairn, H Liu, JL Paluh, B Yang, JS Dordick, KW Moremen, RJ Linhardt. 2013. Structural remodeling of proteoglycans upon retinoic acid-induced differentiation of NCCIT cells. Glycoconj J 30: 497-510. PMID:23053635

JH Prestegard, DA Agard, KW Moremen, LA Lavery, LC Morris, K Pederson. 2013. Sparse Labeling of Proteins: Structural Characterization from Long Range Constraints. J. Magnetic Resonance submitted: -.

F Zhang, HA Moniz, E Condac, KW Moremen, L Wang, RJ Linhardt. 2013. Impact of GFP tagging on Robo1-heparin interactions. Biochem Biophys Res Comm submitted: -.

M Nasirkenari, MB Jones, CC Collins, Z Gao, KW Moremen, M Nemeth, JYT Lau. 2013. Hematopoiesis is Regulated by a Systemic Glycan-Biosynthetic Enzyme, the ST6Gal-l Sialytransferase. Proc Natl Acad Sci USA submitted: -.

A Magalhaes, R Marcos-Pinto, AV Nairn, M dela Rosa, R Ferreira, J Gomes, N Marcos, MR Santos, J Bugaytsova, C Figueiredo, M Dinis-Ribeiro, F Carneiro, KW Moremen, T Boren, L David, CA Reis. 2013. Helicobacter pylori chronic infection switches human gastric cells glycosylation pathways. J. Clin. Invest. Submitted: -.

P. Zhao, A.V. Nairn, S. Hester, K.W. Moremen, R.M. ORegan, G. Oprea, L. Wells, M. Pierce, K.L. Abbott. 2012. Proteomic identification of glycosylphosphatidylinositol anchor-dependent membrane proteins elevated in breat carcinoma. J.Biol Chem. 287: 25230-25240. PMID:22654114

A.W. Barb, L. Meng, Z. Gao, R.W. Johnson, K.W. Moremen, J.H. Prestegard. 2012. NMR charcterization of immunoglobulin G Fc Glycan motion on enzymatic sialylation. Biochemistry 51: 4618-4626. PMID:22574931

D.C. Kraushaar, S. Rai, E. Condac, A.V. Nairn, S. Zhang, Y. Yamaguchi, K. Moremen, S. Dalton, L. Wang. 2012. Heparan sulfate facilitates FGF and BMP signaling to drive mesoderm differentiation of mouse embryonic stem calls. J. Biol. Chem. 287: 22691-22700. PMID:22556407

A.V. Nairn, K. Aoki, M. Dela, M. Porterfield, J.M. Lim, M. Kulik, J.M. Pierce, L. Wells, S. Dalton, M. Tiemeyer, K.W. Moremen. 2012. Regulation of glycan structures in murine embryonic stem cells: Combined transcript profiling of glycan-related genes and glycan structural analysis. J. Biol. Chem. 287: 37835-37856. PMID:22988249

K.W. Moremen, M. Tiemeyer, A.V. Nairn. 2012. Vertebrate protein glycosylation: diversity, synthesis and function. Nat. Rev. Mol. Cell Biol. 13: 448-462. PMID:22722607

L. Gasimli, H.E. Stansfield, A.V. Nairn, H. Liu, J.L. Paluh, B. Yang, J.S. Dordick, K.W. Moremen, R.J. Linhardt. 2012. Structuralremodeling of proteoglycans upon retinoic acid-induced differentiation of NCCIT cells. Glycoconj J. Oct. 2: -. PMID:23053635

H.B. Guo, A. V. Nairn, M. Dela Rosa, T. Nagy, S. Zhao, K. Moremen, M. Pierce. 2012. Transcriptional regulation of the protocadherin beta cluster during Her-2 induced mammary tumorigenesis results from alter N-glycan branching. J. Biol. Chem. 287: 24941-24954. PMID:22665489

A.C. Petrey, H. Flanagan-Steet, S. Johnson, X. Fan, M. Dela Rosa, M.E. Haskins, A.V. Nairn, K.W. Moremen, R. Steet. 2012. Excessive activity of cathepsin K is associated with cartilage defects in a zebrafish model of mucolipidosis II. . Dis Model Mech 5: 177-190. PMID:22046029

H. Park, C.A. Haynes, A.V. Nairn, M. Kulik, S. Dalton, K. Moremen, A.H. Merrill. 2010. Transcript profiling and lipidomic analysis of ceramide subspecies in mouse embryonic stem cells and embryoid bodies. J. Lipid Res. 51: 480-489. PMID:19786568

Y. Zhu, M.D. Suits, A.J. Thompson, S. Chavan, Z. Dinev, C. Dumon, N.L. Smith, K. Moremen, Y. Xiang, A. Siriwardena, S.J. Williams, H.J. Gilbert, G.J. Davies. 2010. Mechanistic insights into a Ca2+-dependent family of a-mannosidases in a human gut symbiont. Nature Chem. Biol. 6: 125-132. PMID:20081828

R. Orlando, J.M. Lim, J.A. Atwood, P.M. Angel, M. Feng, G. Alvarez-Manilla, K.W. Moremen, W.S. York, M. Tiemeyer, J.M. Pierce, S. Dalton, R.L. Wells. 2009. IDAWG: Metabolic incorporation of stable isotope labels for quantitative glycomics of cultured cells. J. Proteome Res. 8: 3816-3823. PMID:19449840

D.J. Termine, K.W. Moremen, R.N. Sifers. 2009. The mammalian UPR boosts glycoprotein ERAD by suppressing the down-regulation of ER mannosidase I. J. Cell Sci. Suppl. 22: 976-984. PMID:19258393

H.B. Guo, A. Vandersall-Nairn, K. Harris, M. Randolph, G. Alvarez-Manilla, K. Moremen, M. Pierce. 2008. Loss of expression of N-acetylglucosaminyltransferase Va results in altered gene expression of glycosyltransferases and galectins. FEBS Lett. 582: 527-535. PMID:18230362

W.E. Zhong, D.A. Kuntz, B. Ember, H. Singh, K.W. Moremen, D.R. Rose, G.-J. Boons. 2008. Probing the substrate specificity of Golgi alpha-mannosidase II by use of synthetic oligosaccharides and a catalytic nucleophile mutant. J. Am. Chem. Soc. 130: 8975-8983. PMID:18558690

K.L. Abbott, A.V. Nairn, E.M. Hall, M.B. Horton, J.F. McDonald, K.W. Moremen, D.M. Dinulescu, M. Pierce. 2008. Focused glycomic analysis of the N-linked glycan biosynthetic pathway in ovarian cancer. Proteomics 8: 3210-3220. PMID:18690643

A.V. Nairn, W.S. York, K. Harris, E.M. Hall, J.M. Pierce, K.W. Moremen. 2008. Regulation of glycan structures in animal tissues: transcript profiling of glycan-related genes. J. Biol. Chem. 283: 17298-17313. PMID:18411279

M.A. Macnaughtan, F. Tian, S. Liu, L. Meng, S. Park, P. Azadi, K.W. Moremen, J.H. Prestegard. 2008. 13C-cialic acid labeling of glycans on glycoproteins using ST6Gal-1. J. Am Chem. Socl 130: 11864-11865. PMID:18700760

S. Liu, A. Venot, L. Meng, F. Tian, K.W. Moremen, G.J. Boons, J.H. Prestegard. 2007. Spin-labeled analogs of CMP-NeuAc as NMR probes of the alpha-2-6-sialyltransferase ST6Gal I. Curr. Opinion Chem. Biol. 14: 409-418. PMID:17462576

Y. Wu, D.J. Termine, M.T. Swulius, K.W. Moremen, R.N. Sifers. 2007. Human endoplasmic reticulum mannosidase I is subject to regulated proteolysis. J. Biol. Chem. 282: 4841-4849. PMID:17166854

A.V. Nairn, A. Kinoshita-Toyoda, H. Toyoda, J. Xie, K. Harris, S. Dalton, M. Kulik, J.M. Pierce, T. Toida, K.W. Moremen, R.J. Linhardt. 2007. Glycomics of proteoglycan biosynthesis in murine embryonic stem cell differentiation. J. Proteome Res. 6: 4374-4387. PMID:17915907

T.O. Akama, H. Nakagawa, N.-K. Wong, M. Sutton-Smith, A. Dell, J. Nakyama, S. Nishimura, A. Pai, K.W. Moremen, J.D. Marth, M.N. Fukuda. 2006. Essential and mutually compensatory roles of a-mannosidase and a-mannosidase IIx in N-glycan processing in vivo in mice. Proc. Natl. Acad. Sci. USA 103: 8983-8988. PMID:16754854

S.W. Mast, K.W. Moremen. 2006. Family 47 a-mannosidases in N-glycan processing. Methods Enzymol. 415: 31-46. PMID:17116466

K.W. Moremen, M. Molinari. 2006. N-linked glycan recognition and processing: the molecular basis of endoplasmic reticulum quality control. Curr. Opin. Struct. Biol. 16: 592-599. PMID:16938451

S.W. Mast, K. Diekman, K. Karaveg, A. Davis, R.N. Sifers, K.W. Moremen. 2005. Human EDEM2, a novel homolog of family 47 glycosidases, is involved in the ER-associated degradation of glycoproteins. Glycobiology 15: 421-436. PMID:15537790

K. Karaveg, A. Siriwardena, W. Tempel, Z.-J. Liu, J. Glushka, B.-C. Wang, K.W. Moremen. 2005. Mechanism of Class 1 (glycosylhydrolase family 47) a-mannosidases involved in N-glycan processing and endoplasmic reticulum quality control. J. Biol. Chem. 280: 16197-16207. PMID:15713668

A. Siriwardena, H. Strachan, S. El-Daher, G. Way, B. Winchester, J. Glushka, K. Moremen, G.J. Boons. 2005. Potent and selective inhibition of Class II a-D-mannosidase activity by a bicyclic sulfonium salt. Chembiochem. 6: 845-848.

K. Karaveg, K.W. Moremen. 2005. Energetics of substrate binding and catalysis by Class 1 (glycosylhydrolase family 47) a-mannosidases involved in N-glycan processing and endoplasmic reticulum quality control. J. Biol. Chem. 280: 29837-29848. PMID:15911611

C.T. Parker, L. Meng, L.H. Stanton, R.E. Collins, S.W. Mast, X. Yi, H. Strachan, K.W. Moremen. 2005. Characterization of a human core-specific lysosomal a1,6-mannosidase involved in N-glycan catabolism. J. Biol. Chem. 280: 37204-37216. PMID:16115860

K.W. Herring, K. Karaveg, K.W. Moremen, W.H. Pearson. 2005. A practical synthesis of kifuensine analogues as inhibitors of endoplasmic reticulum a-mannosidase I. J. Org. Chem. 70: 9892-9904. PMID:16292820

J.K. Lee, L.G. Baum, K. Moremen, M. Pierce. 2004. The X-lectins: A new family with homology to the Xenopus laevis oocyte lectin XL-35. Glycoconjugate J. 21: 443-450.

W. Tempel, K. Karaveg, Z.J. Liu, J.K.C. Rose, B.C. Wang, K.W. Moremen. 2004. Structure of mouse Golgi alpha-mannosidase IA reveal the molecular basis for stubstrate specificity among class 1 (family 47 blycosylhydrolase) alpha,2-mannosidases. J. Biol. Chem. 279: 29774-29784. PMID:15102839

B. Li, S.P. Kawatkar, G.S. Strachan, H. Strachan, R.J. Woods, A. Siriwardena, K.W. Moremen, G.J. Boons. 2004. Inhibition of Golgi mannosidase II with mannostatin A analogues: synthesis, biological evaluation, and structure-activity relationship studies. Chem. BioChem. 5: 1220-1227. PMID:15368573

Y. Wu, K.W. Moremen, R.N. Sifers. 2003. Elucidation of the molecular logic that preferentially targets misfolded alpha1-antitrypsin for intracellular degradation. Proc. Natl. Acad. Sci. USA 100: 8229-8234. PMID:12815101

M. Kaneko, M. Kamar, W.-J. Zhang, J.-K. Lee, K. Moremen, M. Pierce. 2003. A novel N-acetylglucosaminyltransferase, GnT-VB, that synthesizes N-linked b(1,6) branched glycans. FEBS Lett. 554: 515-519. PMID:14623122

T.O. Akama, H. Nakagawa, K. Sugihara, S. Narisawa, C. Ohyama, S. Nishimura, D.A. O'Brien, K.W. Moremen, J.L. Millan, M.N. Fukuda. 2002. Germ cell survival through carbohydrate-mediated interaction with Sertoli cells. Science 295: 124-127. PMID:11778047

C.M. Cabral, Y. Liu, K.W. Moremen, R.N. Sifers. 2002. Organizational diversity among distinct glycoprotein ER-associated degradation programs. Mol. Biol. Cell 13: 2639-2650. PMID:12181335

K. Moremen. 2002. Golgi a-mannosidase II deficiency in vertebrate systems: implications for asparagine-linked oligosaccharide processing in mammals. Biochem. Biophys. Acta. 1573: 225-235. PMID:12417404

J.-K. Lee, M. Pang, J. Schnee, L. Baum, K. Moremen, M. Pierce. 2001. Human homologs of the Xenopus oocyte cortical granule lectin XL35. Glycobiology 11: 65-73. PMID:11181563

M. Oh-Eda, H. Nakagawa, T.O. Akayama, K. Lowitz, M. Misago, K.W. Moremen, M.N. Fukuda. 2001. Overexpression of the Golgi-localized enzyme a-mannosidase IIx in Chinese hamster ovary cells results in the conversion of hexamannosyl-N-acetylchitobiose to tetramannoseyl-N-acetylchitobiose in the N-glycan processing pathway. Eur. J. Biochem. 268: 1280-1288. PMID:11231279

C.A. Rivera-Marrero, J.D. Ritzenthaler, J. Roman, K.W. Moremen. 2001. Molecular cloning and expression of an a-mannosidase gene in Mycobacterium tuberculosis. Microbiol. Pathog. 30: 9-18. PMID:11162181

Z. Kawar, K. Karaveg, K.W. Moremen, D.L. Jarvis. 2001. Insect cells encode a class II alpha-mannosidase with unique properties. J. Biol. Chem. 276: 16335-16340. PMID:11279010

F. VAllee, K. Karaveg, A. Herscovics, K.W. Moremen, P.L. Howell. 2000. Structural basis for catalysis and inhibition of N-glycan processing class I alpha 1,2,-mannosidases. J. Biol. Chem. 275: 41287-41298. PMID:10995765

D.S. Gonzalez, Y. Kagawa, K.W. Moremen. 1999. Isolation and characterization of the gene encoding the mouse broad specificity lysosomal alpha-mannosidase. Biochim. Biophys. Acta 1445: 177-183. PMID:10209272

D.S. Gonzalez, K. Karaveg, A.S. Vandersall-Nairn, A. Lal, K.W. Moremen. 1999. Identification, expression, and characterization of a cDNA encoding human endoplasmic reticulum mannosidase I, the enzyme that catalyzes the first mannose trimming step in mammalian Asn-linked oligosaccharide biosynthesis. J. Biol. Chem. 274: 21375-21386. PMID:10409699

F. Valee, A. Lal, K.W. Moremen, P.L. Howell. 1999. Purification, crystallization and preliminary X-ray crystallographic analysis of recombinant murine Golgi mannosidase IA, a Class I alpha-mannosidase involved in Asn-linked oligosaccharide maturation. Acta Crystallog. D55: 571-573.

S.A. Igdoural, A. Herscovics, A. Lal, K.W. Moremen, C.R. Morales, L. Hermo. 1999. a-Mannosidases involved in N-glycan processing show cell specificity and distinct subcompartmentalization with the Golgi apparatus of cells in the testis and epididymus. Eur. J. Cell Biol. 78: 441-452. PMID:10472797

A. Lal, P. Pang, S. Kalelkar, P.A. Romero, A. Herscovics, K.W. Moremen. 1998. Substrate specificities of recombinant murine Golgi a-1,2-mannosidases IA and IB and comparison with endoplasmic reticulum and Golgi processing a-1,2-mannosidases. Glycobiology 8: 981-995. PMID:9719679

A. Vandersall-Nairn, K. O'Brien, R.K. Merkle, T.N. Oeltmann, K.W. Moremen. 1998. Cloning, expression, purification, and characterization of the acid alpha-mannosidase from Trypanosoma cruzi. Glycobiology 8: 1183-1194. PMID:9858640

D. Chui, M. Oh-Eda, Y.-F. Liao, K. Panneerselvam, A. Lal, W.W. Marek, H.H. Freeze, K.W. Moremen, M.N. Fukuda, J.D. Marth. 1997. Alpha-mannosidase-II deficiency results in dyserythropoiesis and unveils an alternate pathway in oligosaccharide biosynthesis. Cell 90: 157-167. PMID:9230311

S. Howard, C. Braun, J. McCarter, K.W. Moremen, Y.-F. Liao, S.G. Withers. 1997. Human lysosomal and jack bean a-mannosidases are retaining glycosidases. Biochem. Biophys. Res. Commun. 238: 896-898. PMID:9325188

J.-K. Lee, P. Buckhaults, C. Wilkes, M. Teilhet, K. Moremen, M. King, M. Pierce. 1997. Cloning and expression of the Xenopus laevis oocyte lectin and characterization of its mRNA levels during early development. Glycobiology 7: 367-372. PMID:9147045

R.K. Merkle, Y. Zhang, P.J. Ruest, A. Lal, Y.-F. Liao, K.W. Moremen. 1997. Cloning, expression, purification, and characterization of the murine lysosomal acid a-mannosidase. Biochim. Biophys. Acta 1336: 132-146. PMID:9305783

D.L. Jarvis, D.A. Bohlmeyer, Y.-F. Liao, K.K. Lomax, R.K. Merkle, K.W. Moremen. 1996. Isolation and characterization of a class II a-mannosidase cDNA from lepidopteran insect cells. Glycobiology 7: 113-127.

Y.-F. Liao, A. Lal, K.W. Moremen. 1996. Cloning, expression, purification, and characterization of the human broad specificity lysosomal acid a-mannosidase. J. Biol. Chem. 271: 28348-28358. PMID:8910458

M. Misago, Y.-F. Liao, S. Kudo, M.-G. Mattei, K.W. Moremen, M.N. Fukuda. 1995. Molecular cloning and expression of cDNAs encoding human a-mannosidase II and a novel a-mannosidase IIx isozyme. Proc. Natl. Acad. Sci. USA 92: 11766-11770. PMID:8524845

P.A. Prieto, P. Mukerjit, B. Kelder, R. Erney, D. Gonzalez, J.S. Yun, D.F. Smith, K.W. Moremen, C. Nardelli, M. Pierce, Y. Li, X. Chen, T.E. Wagner, R.D. Cummings, J.J. Kopchick. 1995. Remodeling of mouse milk glycoconjugates by transgenic expression of a human glycosyltransferase. J. Biol. Chem. 270: 29515-29519.

A. Herscovics, J. Schneikert, A. Athanassiadis, K.W. Moremen. 1994. Isolation of a mouse Golgi mannosidase cDNA, a member of a gene family conserved from yeast to mammals. J. Biol. Chem. 269: 9872-9881.

A. Lal, J.S. Schutzbach, W.T. Forsee, P.J. Neame, K.W. Moremen. 1994. Isolation and expression of murine and rabbit a1,2-mannosidase involved in the processing of asparagine-linked oligosaccharides. J. Biol. Chem. 269: 9864-9871.

K.W. Moremen, R.B. Trimble, A. Herscovics. 1994. Glycosidases of the asparagine-linked oligosaccharide processing pathway. Glycobiology 4: 113-125.

T. Oeltman, C. Carter, R. Merkle, K. Moremen. 1994. The a-mannosidase of Trypanosoma cruzi: structure and function. Braz. J. Med. Biol. Res. 27: 483-488.

C. Abeijon, K. Yanigasawa, E. Mandon, A. Hausler, K. Moremen, C.B. Hirschberg, P.W. Robbins. 1993. Guanosine diphosphatase is required for protein and lipid glycosylation in the Golgi lumen of Saccharomyces cerevisiae. J. Cell Biol. 122: 307-323.

R. Sasisekharan, M. Bulmer, K.W. Moremen, C.L. Cooney, R. Langer. 1993. Cloning and expression of heparinase I gene from Flavobacterium heparinum. Proc. Natl. Acad. Sci. USA 90: 3660-3664.

A. Velasco, K.W. Moremen, L. Hendricks, D.R.P. Tulsiani, O. Touster, M.G. Farquhar. 1993. Cell-type dependent variations in the subcellular distribution of a-mannosidase IA and II. J. Cell Biol. 122: 39-51.

K.W. Moremen, P.W. Robbins. 1991. Isolation, characterization, and expression of cDNA encoding murine a-mannosidase II, a Golgi enzyme that controls conversion of high mannose to complex N-glycans. J. Cell Biol. 115: 1521-1534.

K.W. Moremen, P.W. Robbins, O. Touster. 1991. Novel purification of the catalytic domain of Golgi mannosidase II: Characterization and comparison with the intact enzyme. J. Biol. Chem. 266: 16876-16885.

C. Zuber, J. Roth, T. Mistelli, A. Nakano, K. Moremen. 1991. DS 28-6, a temperature-sensitive mutant of Chinese hamster ovary cells, expresses the key phenotypic changes associated with Brefeldin A treatment. Proc. Natl. Acad. Sci. USA 88: 9818-9822.

J. Bischoff, K.W. Moremen, H. Lodish. 1990. Isolation, characterization, and expression of a cDNA encoding the rat liver endoplasmic reticulum a-mannosidase. J. Biol. Chem. 265: 17110-17117.

M.N. Fukuda, K.A. Masri, A. Dell, L. Luzzato, K.W. Moremen. 1990. Incomplete synthesis of N-glycans in congenital dyserythropoetic anemia type II (HEMPAS) caused by a gene defect encoding a-mannosidase II. Proc. Natl. Acad. Sci. USA 87: 7443-7447.

K.W. Moremen. 1989. Isolation of a rat liver Golgi mannosidase II clone by mixed oligonucleotide-primed amplification of cDNA. Proc. Natl. Acad. Sci. USA 86: 5276-5280.

K.W. Moremen, O. Touster. 1986. Topology of mannosidase II in rat liver Golgi membranes and release of the catalytic domain by selective proteolysis. J. Biol. Chem. 261: 10945-10951.

K.W. Moremen, O. Touster. 1985. Biosynthesis and modification of Golgi mannosidase II in HeLa and 3T3 cells. J. Biol. Chem. 260: 6654-6662.

FM Zhang, HA Moniz, KW Moremen, L. Wang, RJ Linhardt. 0. Analysis of the impact of GFP tagging on protein-heparin interaction by surface Plasmon resonance. Glycoconj. J. In press.

Book Chapters

AV Nairn, KW Moremen. 2015. Glycotranscriptomics in Glycoscience: Biology and Medicine (N. Taniguchi, T. Endo, G. Hart, P. Seeberger, C.-H. Wang). In: , pp. 1475-1482. Springer-Verlag, Tokyo.

A.V. Nairn, K.W. Moremen. 2014. Glucosidase, alpha neutral AB; glucosidase II subunit beta (GANAB, PRKCSH, alpha-glucosidase II). In: Handbook of Glycosyltransferases and Related Genes, 2nd edition (Taniguchi N. ed) Springer-Verlag, Tokyo, In press.

A.V. Nairn, K.W. Moremen. 2014. Mannosyl-oligosaccharide glucosidase (Glucosidase I, MOGS). In: Handbook of Glycosyltransferases and Related Genes, Vol.2nd edition. Springer-Verlag, Tokyo.

K.W. Moremen, A.V. Nairn. 2014. Mannosidase, alpha, class 2a1 (MAN2A1, Golgi alpha-mannosidase II). In: Handbook of Glycosyltransferases and Related Genes, 2nd edition Springer-Verlag, Tokyo, In press.

K.W. Moremen, A.V. Nairn. 2014. Mannosidase, alpha, class 1 (MAN1A1) (Golgi alpha-, class 1 (MAN1A1 (Golgi alpha-mannosidase 1A), Man1A2 (Golgi alpha-mannosidase 1B), MAN1B1 (ER alpha-mannosidase I), MAN1C1 (Golgi alpha-mannosidase 1C). In: Handbook of Glycosyltransferases and Related Genes, 2nd edition, Vol.in press. Springer-Verlag, Tokyo.

AV Nairn, KW Moremen. 2014. Glucosidase, alpha neutral AB; glucosidase II subunit beta (GANAB, PRKCSH, alpha-glucosidase II). In: Handbook of Glycosyltransferases and Related Genes, 2nd edition, Vol.in press. Springer-Verlag, Tokyo.

K.W. Moremen, A.V. Nairn. 2013. Mannosidase, alpha, class 1 (MAN1A1 (golgi alpha-mannosidase lA), Man1A2 (Golgi alpha-mannosidase IB), MAN1B1 (ER alpha-mannosidase 1), MAN1C1 (Golgi, alpha-mannosidase IC). . In: Handbook of Glycosyltransferases and Related Genes, 2nd edition (Taniguchi N, ed) Springer-Verlag, Tokyo, In press.

A.V. Nairn, K.W. Moremen. 2013. Mannosyl-oligosaccharide glucosidase (Glucosidase I, MOGS) . In: Handbook of Glycosylransferases and Related Genes, 2nd edition (Taniguchi N, ed.) Springer-Verlag, Tokyo, In press.

A. Nairn, K. Moremen. 2009. Glycotranscriptomics. In: Handbook of Glycomics (R.D. Cummings and J.M. Pierce, eds.), pp. 81-135. Academic Press.

K.W. Moremen. 2001. a-Mannosidase II. In: Handbook of Glycosyltransferases and Related Genes (N. Taniguchi and M. Fukuda, eds.), pp. 600-606. Springer-Verlag, Tokyo.

K.W. Moremen. 2001. Mannosidases in asparagine-linked oligosaccharide processing and catabolism. In: Oligosaccharides in Chemistry and Biology: A Comprehensive Handbook, Vol II, Part 1 (B. Ernst, G.Hart, and P. Sindy, eds.), pp. 81-117. Wiley and Sons Publishers.

R.B. Trimble, K.W. Moremen, A. Herscovics. 1995. Glycosidases of the endoplasmic reticulum and Golgi apparatus. In: Guidebook to the Secretory Pathway (J.A. Rothblatt, T. Stevens, and P. Novick, eds.) . Sambrook and Tooze Scientific Publishers.

K.W. Moremen, O. Touster. 1987. Mannosidases in mammalian glycoprotein processing. In: Protein Transfer and Organelle Biogenesis (R.C. Das and P.W. Robbins, eds.), pp. 209-240. Academic Press.

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