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Michael Tiemeyer
Professor of Biochemistry and Molecular Biology

Mechanisms that regulate the expression, function, and structure of tissue-specific glycans

Telephone: 706-542-2740
Fax: 706-542-4412

Short Biography
Research Interests
Lab-personal web site

Short Biography:

Dr. Tiemeyer received his B.A. in biology in 1982 and his Ph.D. in neuroscience in 1989 from The Johns Hopkins University. He was a Helen Hay Whitney postdoctoral fellow in developmental neurobiology at the University of California at Berkeley. Prior to joining the CCRC faculty, Dr. Tiemeyer was a faculty member in cell biology at Yale University School of Medicine and Director of Biochemical and Clinical Analytics and New Methods Development at Glyko/Biomarin, Inc. Full publications: 27.

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Research Interests:

The surfaces of all eukaryotic cells are richly endowed with a diverse array of complex glycoconjugates. Therefore, carbohydrate moieties linked to protein, lipid, and glycosaminoglycan form the interfaces at which cell-cell interactions occur. Consistent with their subcellular location and structural diversity, specific oligosaccharides function as cell-surface tags that allow cells to appropriately interact with each other and with their local environment. In fact, cell surface carbohydrates are among the most discriminating markers for cellular differentiation and pathogenesis. We utilize genetic, molecular, and chemical techniques in vertebrate (mouse) and insect (Drosophila) model systems to study two aspects of carbohydrate expression. First, we investigate the influence of cell surface carbohydrates on development of the nervous system. We identified and characterized a novel carbohydrate binding protein (Gliolectin) that mediates the fidelity of axon pathfinding early in neural development. Second, we study mechanisms that control tissue- and stage-specific oligosaccharide expression. We discovered that a member of the Toll-like receptor family (Tollo) influences tissue-specific glycosylation through cell-cell communication. Our results have implications for facilitating regeneration of axon pathways in the nervous system, for understanding innate immunity and tissue surveillance, and for controlling the cellular changes that precede tumor metastasis.

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Publications: Author's Last Name: Tiemeyer

Journal Articles
Book Chapters are listed at the bottom of this page.

T Zhou, NA Dorie-Rose, C Cheng, GBE Stewart-Jones, GY Chuang, M Chambers, AR Druz, H Geng, K McKee, YD Kwon, S O'Dell, M Sastry, SD Schmidt, K Xu, L Chen, RE Chen, MK Louder, M Pancera, TG Wanninger, B Zhang, A Zheng, SK Farney, KE Foulds, IS Georgiev, MG Joyce, T Lemmin, S Narpala, R Rawl, C Soto, JP Todd, CH Shen, Y Tsybovsky, Y Yang, P Zhao, BF Haynes, L Stamatatos, M Tiemeyer, L Wells, DG Scorpio, L Shapiro, AB McDermott, JR Mascola, PD Kwong. 2017. Quantification of the Impact of the HIV-1-Glycan Shield on Antibody Elicitation. Cell Rep. Apr 25; 19(4): 719-732. PMID:28445724

M. Tiemeyer, K. Aoki, J. Paulson, R.D. Cummings, W.S. York, N.G. Karlsson, F. Lisacek, N.H. Packer, M.P. Campbell, N.P. Aoki, A. Fujita, M. Matsubara, D. Shinmachi, S. Tsuchiya, I. Yamada, M. Pierce, R. Ranzinger, H. Narimatsu, K.F. Aoki-Kinoshita. 2017. GlyTouCan: An accessible glycan structure repository. Glycobiology 27: 915-919. PMID:28922742

WB Struwe, S. Agravat, KF Aoki-Kinoshita, MP Campbell, CE Costello, A. Dell, T Feizi, SM Haslam, NG Karlsson, KH Khoo, D. Kolarich, Y. Liu, R. McBride, MV Novotny, NH Packer, JC Paulson, E. Rapp, R. Ranzinger, PM Rudd, DF Smith, M. Tiemeyer, L. Wells, WS York, J. Zaia, C Kettner. 2016. The minimum information required for a glycomics experiment (MIRAGE) project: sample preparation guidelines for reliable reporting of glycomics datasets. Glycbiology Sept 26(9): 907-910. PMID:27654115

N Mehta, M Porterfield, WB Struwe, C Heiss, P Azadi, PM Rudd, M Tiemeyer, K Aoki. 2016. Mass Spectrometric Quantification of N-Linked Glycans by Reference to Exogenous Standards. Journal of Proteome Research 15(9): 2969-2980. PMID:27432553

Al Jadda, MP Porterfield, R. Bridger, C. Heiss, M. Tiemeyer, L. Wells, JA Miller, WS York, R. Ranzinger. 2015. EUROCarbDB(CCRC): a EUROCarbDB node for storing glycomics standard date. Bioinformatics Jan 15;31(2): 242-245. PMID:25217575

H Allam, K Aoki, BB Benigno, JF McDonald, SG Mackintosh, M Tiemeyer, KL Abbot. 2015. Glycomic Analysis of Membrane Glycoproteins with Bisecting Glycosylation from Ovarian Cancer Tissues Reveals Novel Structures and Functions. J Proteome Res 14: 434-446. PMID:25437919

T Kiwamoto, T Katoh, CM Evans, WJ Janssen, ME Brummet, SA Hudson, Z Zhu, M Tiemeyer, BS Bochner. 2015. Endogenous airway mucins carry glycans that bind Siglec-F and induce eosinophil apoptosis. J Allergy Clin Immunol 135: 1329-1340. PMID:25497369

R Bammens, N Mehta, V Race, F Foulquier, J Jaeken, M Tiemeyer, R Steet, G Matthijs, H Flanagan-Steet. 2015. Abnormal Cartilage Development and Altered N-Glycosylation in Tmem165-Deficient Zebrafish Mirrors the Phenotypes Associated with TMEM165-CDG. Glycobiology 25: 669-682. PMID:25609749

CA Dwyer, T Katoh, M Tiemeyer, RT Matthews. 2015. Neurons and glia modify receptor protein-tyrosine phosphaase ζ (RPTPζ)/phosphacan with cell-specific O-manosyl glycans in the developing brain . JBC 290: 10256-10273. PMID:25737452

S Kurz, K Aoki, C Jin, NG Karlsson, M Tiemeyer, I Wilson, K Pashinger. 2015. Targetted release and fractionation reveal glucuronylated and sulfated N- and O-glycans in larvae of dipteran insects. J Proteomics 126: 172-188. PMID:26047717

L Boccuto, K Aoki, H Flanagan-Steet, CF Chen, X Fan, F Bartel, M Petukh, A Pittman, R Saul, A Chaubey, E Alexov, M Tiemeyer, R Steet, CE Schwartz. 2014. A mutation in a ganglioside biosynthetic enzyme, ST3GAL5, results in salt & pepper syndrome, a neurocutaneous disorder with altered glycolipid and glycoprotein glycosylation . Hum Mol Genet 23(2): 418-433. PMID:24026681

WS York, S. Agravat, KF Aoki-Kinoshita, R. McBride, MP Campbell, CE Costello, A. Dell, T Feizi, SM Haslam, N.G. Karlsson, KH Khoo, D. Kolarich, Y. Liu, M.V. Novotny, NH Packer, JC Paulson, E. Rapp, R Razinger, PM Rudd, DF Smith, WB Struwe, M. Tiemeyer, L. Wells, J. Zaia, C Kettner. 2014. MIRAGE: the minimum information required for a glycomics experiment. Glycobiology 24(5): 402-406. PMID:24653214

M Porterfield, P Zhao, H Han, J Cunningham, K Aoki, DD Von Hoff, MJ Demeure, JM Pierce, M Tiemeyer, L Wells. 2014. Discrimination between Adenocarcinoma and normal pancreatic ductal fluid by proteomic and glycomic analysis. J Proteome Res Feb 7; 13(2): 395-407. PMID:24328148

G Kunduri, C Yuan, V Parthibane, KM Nyswaner, RR Kanwar, K Nagashima, SG Britt, N Mehta, V Kotu, M Porterfield, M Tiemeyer, PJ Dolph, U Acharya, JK Acharya . 2014. Phosphatidic acid phospholipase A1 mediates ER-Golgi transmit of a family of G protein-coupled receptors. J Cell Biol 206(1): 79-95. PMID:25002678

K. Talabnin, K. Aoki, P. Saichua, S. Wongkham, S. Kaewkes, G.J. Boons, B. Sripa, M. Tiemeyer. 2013. Stage-specific expression and antigenicity of glycorpotein glycans isolated from the human liver fluke, Opisthorchis viverrini. Int. J. Parasitol 43: 37-50. PMID:23174105

Y. Xiang, X. Zhang, D.B. Nix, T. Katoh, K. Aoki, M. Tiemeyer, Y. Wang. 2013. Regulation of protein glycosylation and sorting by the Golgi matrix proteins GRASP55/65. Nature Commun. 4: 1659-. PMID:23552074

T. Kiwamoto, T. Katoh, M. Tiemeyer, B.S. Bochner. 2013. The role of lung epithelial ligands for Siglec-8 and Siglec-F in eosinophilic inflammation. Curr. Opin. Allergy Clin. Immunol. 13: 106-111. PMID:23160308

T. Katoh, M. Tiemeyer. 2013. The Ns and Os of Drosophila glycoprotein glycobiology. Glycoconjugate J. 30: 57-66. PMID:22936173

M. Porterfield, Z Peng, H Haiyong, J Cunningham, K. Aoki, D Von, J Demeure, M. Pierce, M. Tiemeyer, L. Wells. 2013. Discrimination between Adenocarcinoma and Normal Pancreatic Ductal Fluid by Proteomic and Glycomic Analysis. J. Proteome Res. In press.

A.V. Nairn, K. Aoki, M. Dela, M. Porterfield, J.M. Lim, M. Kulik, J.M. Pierce, L. Wells, S. Dalton, M. Tiemeyer, K.W. Moremen. 2012. Regulation of glycan structures in murine embryonic stem cells: Combined transcript profiling of glycan-related genes and glycan structural analysis. J. Biol. Chem. 287: 37835-37856. PMID:22988249

K.W. Moremen, M. Tiemeyer, A.V. Nairn. 2012. Vertebrate protein glycosylation: diversity, synthesis and function. Nat. Rev. Mol. Cell Biol. 13: 448-462. PMID:22722607

G. Kadirvel, S.A. Machado, C. Korneli, E. Collins, P. Miller, K.N. Bess, K. Aoki, M. Tiemeyer, N. Bovin, D.J. Miller. 2012. Porcine sperm bind to specific 6-sialylated biantennary glycans to form the oviduct reservoir. Biol. Reprod. 87: 147-. PMID:23115267

C. Geisler, V. Kotu, M. Sharrow, D. Rendic, G. Poltl, M. Tiemeyer, I.B. Wilson, D.L. Jarvis. 2012. The Drosophila neurally altered carbohydrate mutant has a defective Golgi GDP-fucose transporter. J. Biol. Chem. 287: 29599-29609. PMID:22745127

E.R. Sturgill, K. Aoki, P.H. Lopez, D. Colacurcio, K. Vajn, I. Lorenzini, S. Majic, W.H. Yang, M. Heffer, M. Tiemeyer, J.D. Marth, R.L. Schnaar. 2012. Biosynthesis of the major brain gangliosides GD1a and GT1b. Glycobiology 22: 1289-1301. PMID:22735313

S.H. Stainaker, K. Aoki, J.M. Lim, M. Porterfield, M. Liu, J.S. Satz, S. Buskirk, Y. Xiong, P. Zhang, K.P. Campbell, H. Hu, D. Live, M. Tiemeyer, L. Wells. 2011. Glycomic analyses of mouse models of congenital muscular dystrophy. J. Biol. Chem. 286: 21180-21190. PMID:21460210

M.C. Reilly, K. Aoki, Z.A. Wang, M.L. Skowyra, M. Williams, M. Tiemeyer, T.L. Doering. 2011. A xylosylphosphotransferase of Cryptococcus neoformans acts in protein O-glycan synthesis. J. Biol. Chem. 286: 26888-26899. PMID:21606487

S. Baas, M. Sharrow, V. Kotu, M. Middleton, K. Nguyen, H. Flanagan-Steet, K. Aoki, M. Tiemeyer. 2011. Sugar-free frosting, a homolog of SAD kinase, drives neural-specific glycan expression in the Drosophila embryo. Development 138: 553-563. PMID:21205799

S.H. Stalnaker, S. Hashmi, J-M Lim, K. Aoki, M. Porterfield, G. Gutierrez-Sanchez, J. Wheeler, J.M. Ervasti, C. Bergmann, M. Tiemeyer, L. Wells. 2010. Site-mapping and characterization of O-glycan structures on alpha-dystroglycan isolated from rabbit skeletal muscle. J. Biol. Chem. 285: 24882-24891. PMID:20507986

S.H. Stalnaker, S. Hashmi, J-M Lim, K. Aoki, M. Porterfield, G. Gutierrez-Sanchez, J. Wheeler, J.M. Ervasti, C. Bergmann, M. Tiemeyer, L. Wells. 2010. Site-mapping and characterization of O-glycan structures on alpha-dystroglycan isolated from rabbit skeletal muscle. J. Biol. Chem. 285: 24882-24891. PMID:20507986

R. Orlando, J.M. Lim, J.A. Atwood, P.M. Angel, M. Feng, G. Alvarez-Manilla, K.W. Moremen, W.S. York, M. Tiemeyer, J.M. Pierce, S. Dalton, R.L. Wells. 2009. IDAWG: Metabolic incorporation of stable isotope labels for quantitative glycomics of cultured cells. J. Proteome Res. 8: 3816-3823. PMID:19449840

J.M. Lim, K. Aoki, P.M. Angel, D. Garrison, D. King, M. Tiemeyer, C. Bergmann, L. Wells. 2009. Mapping glycans onto specific N-linked glycosylation sites of Pyrus communis PGIP redefines the interface for EPG-PGIP interactions. J. Proteome Res. 8: 673-680. PMID:19072240

K.L. Abbott, K. Aoki, J.-M. Lim, M. Porterfield, R. Johnson, R.M. O'Regan, L. Wells, M. Tiemeyer, M. Pierce. 2008. Targeted glycoproteomic identification of biomarkers for human breast carcinoma. J. Proteome Res. 7: 1470-1480. PMID:18271524

L. Nimrichter, M.M. Burdick, K. Aoki, W. Laroy, M.A. Fierro, S.A. Hudson, C.E. Von, R.J. Cotter, B.S. Bochner, M. Tiemeyer, K. Konstantopoulos, R.L. Schnaar. 2008. E-selectin receptors on human leukocytes. Blood 112: 3744-3752. PMID:18579791

K. Aoki, M. Porterfield, S.S. Lee, B. Dong, K. Nguyen, K.H. McGlamry, M. Tiemeyer. 2008. The diversity of O-linked glycans expressed during Drosophila melanogaster development reflects stage-and tissue-specific requirements for cell signaling. J. Biol. Chem. 283: 30385-30400. PMID:18725413

K. Aoki, M. Perlman, J.M. Lim, R. Cantu, L. Wells, M. Tiemeyer. 2007. Dynamic developmental elaboration of N-linked glycan complexity in the Drosophila melanogaster embryo. J. Biol. Chem. 282: 9127-9142. PMID:17264077

P.M. Matani, M. Sharrow, M. Tiemeyer. 2007. Ligand, modulatory, and co-receptor functions of neural glycans. Frontiers of Biosci. 12: 3852-3879. PMID:17485343

K. Koles, J.M. Lim, K. Aoki, M. Porterfield, M. Tiemeyer, L. Wells, V. Panin. 2007. Identification of N-glycosylated proteins from the central nervous system of Drosophila melanogaster. Glycobiology 17: 1388-1403. PMID:17893096

R. Muller, A.J. Hulsmeier, F. Altmann, K. Ten-Hagen, M. Tiemeyer, T. Hennet. 2005. Characterization of mucin-type core-1 b-3 galactosyltransferase homologous enzymes in Drosophila melanogaster. FEBS J. 272: 4295-4305. PMID:16128800

A.S. Seppo, P.M. Matani, M. Sharrow, M. Tiemeyer. 2003. Induction of neuron-specific glycosylation by Tollo/Toll-8, a Drosophila Toll-like receptor expressed in non-neutral cells. Development 130: 1439-1448. PMID:12588858

Book Chapters

M. Sharrow, K. Aoki, S. Baas, M. Poeterfield, M. Tiemeyer. 2009. Genetic and structural analysis of the glycoprotein and glycolipid glycans of Drosophila melanogaster. In: Handbook of Glycomics (R. Cummings and J.M. Pierce, eds.), pp. 329-345. Academic Press.

M. Tiemeyer, S.B. Selleck, J.D. Esko. 2007. Arthropoda. Chapter 24. In: Essentials of Glycobiology (A. Varki, R. Cummings, J. Esko, H. Freeze, G. Hart, and J. Marth, eds.) . Cold Spring Harbor Laboratory Press.

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