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Artur Muszynski
Associate Research Scientist


Structure and function of lipopolysaccharides and cell surface polysaccharides in human bacterial pathogens and plant symbiotic bacteria

E-mail: muszynski@ccrc.uga.edu
Telephone: 706-542-4479
Fax: 706-542-4412

Short Biography
Research Interests
Publications

Short Biography:
Dr. Muszyński is an Associate Research Scientist at the CCRC. He received his M.Sc. degree and his Ph.D. degree in Biology from the University of Silesia, Katowice, Poland, in 1999 and 2004 respectively. In 1998 he was awarded two semesters of Socrates Erasmus scholarship to study advanced biochemistry and microbiology at the Faculty of Science and Department of Microbiology, Aarhus University, Aarhus, Denmark. In 1999 and 2003 he was a visiting scientist at the Research Center Borstel, Leibniz-Center for Medicine and Biosciences Borstel, Germany in the laboratory of Prof. Otto Holst. As a Ph.D. candidate, Dr. Muszyński conducted research on characterization of lipopolysaccharides from pathogenic Yersinia enterocolitica O:3 under supervision of Prof. Dr. Joanna Radziejewska-Lebrecht and Prof. Dr. Otto Holst. In 2005 he joined the group of Dr. Russell Carlson at the Complex Carbohydrate Research Center, University of Georgia for his postdoctoral studies. Currently Dr. Artur Muszyński is an Associate Research Scientist faculty member at the CCRC successfully working on determining the chemical and structural properties of bacterial cell surface glycans and glycolipids including lipopolysaccharide (LPS), lipid A, capsular polysaccharide (CPS), and extracellular polysaccharides (EPS), and other bacterial glycans and elucidating their biological roles in host-microbe interactions. He is also involved in multiple collaborative projects with academia and industry. In addition to his research Dr. Muszyński’s supervises undergraduate students introducing to world of microbial glycobiology.

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Research Interests:
The outer leaflet of the outer membrane of the gram-negative bacterial cell envelope is built of lipopolysaccharide (LPS). The LPS consists of three distinct structural regions; a rather conserved lipid A, a more variable core oligosaccharide (OS), and an even more variable O-antigen polysaccharide (OPS). These regions are known to play important roles in interacting with the defense mechanisms of the host cells. In addition LPS, as well as capsular polysaccharides (CPS) and bacterial extracellular polysaccharides (EPS) are essential for protecting bacteria from environmental stress and in symbiotic interactions.

Determination of chemical and structural properties of these glycan and glycolipid macromolecules is critical in understanding the mechanisms of the host-microbe interactions and, in the case of pathogenic bacteria, in searching for good vaccine candidates. Through past years of my work I was involved in studies on LPS or lipid A from pathogenic, Pseudomonas aeruginosa, Neisseria meningitidis, Neisseria gonorrhoeae, Francisella novicida, Fancisella tularensis, Moraxella catharrhalis, Yersinia pestis, Yersinia enterocolitica, Bulkholderia multivorans, Helicobacter pylori, Campylobacter jejuni, Xyllela fastidiosa, Rimerella antipestifer, Granulibacter bethesdensis and nitrogen fixing Rhizobium leguminosarum, Rhizobium galegae, Sinorhizobium meliloti, Rhizobium etli, Mesorhizobium loti, Herbaspirillum seropedicae, and on peptidoglycan in Micrococcus lutes and Neisseria sp.

My current research projects involve studies on structural and biological properties of Mesorhizobium loti EPS, roles of polysaccharides in biofilm formation, in pathogenic Campylobacter jejuni and Helicobacter pylori, and biochemical studies on lipopolysaccharide of Bradyrhizobium japonicum and its role in legume symbiosis.

These studies utilize various techniques used in biochemistry and microbiology laboratories and analytical techniques, particularly chromatography and mass spectrometry (GPC, GLC-MS, CI-MS, HPLC, TLC, ESI-MS, MALDI-TOF and TOF-TOF MS) and NMR spectroscopy.

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Publications: Author's Last Name: Muszynski

Journal Articles
Book Chapters are listed at the bottom of this page.

J.N. Rapicavoli, B. Blanco-Ulate, A. Muszynski, R. Figueroa-Balderas, A. Morales-Cruz, P. Azadi, J.M. Dobruchowska, C. Castro, D. Cantu, M.C. Roper. 2018. Lipopolysaccharide O-antigen delays plant innate immune recognition of Xylella fastidiosa. Nature Commun. 9: 390-390. PMID:29374171

X Kang, A Kirui, A Muszynski, MCD Widanage, A Chen, P Azadi, P Wang, F Mentink-Vigier, T Wang. 2018. Molecular architecture of fungal cell walls revealed by solid-state NMR. Nat Commun 9(1): 2747-. PMID:30013106

JL Kandler, CL Holley, JL Reimche, V Dhulipala, JT Balthazar, A Muszynski, RW Carlson, WM Shafer. 2016. The MisR response regulator is necessary for intrinsic cationic antimicrobial peptide and amino glycoside resistance in Neisseria gonorrhoeae. Antimicrob Agents Chemother 60: 4690-4700. PMID:27216061

A Muszynski, C Heiss, CT Hjuler, JT Sullivan, SJ Kelly, MB Thygesen, J Stougaard, P Azadi, RW Carlson, CW Ronson. 2016. Structures of exopolysaccharides involved in receptor-mediated perception of Mesorhizobium lori by Lotus japonicus. J. Biol. Chem. doi.org/10.1074/: jbc.m116.743856-.

A Muszynski, C Heiss, CT Hjuler, JT Sullivan, SJ Kelly, MB Thygesen, J Stougaard, P Azadi, RW Carlson, RW Ronson. 2016. Structures of exopolysaccharides involved in receptor-mediated perception of Mesorhizobium lori by Lotus japonicus. J Biol Chem Aug 8: jbc.M116.743856-. PMID:27502279

Y Kawaharada, S Kelly, M Nielsen, H Wibroe, CT Hjuler, K Gysel, A Muszynski, RW Carlson, MB Thygesen, N Sandal, MH Asmussen, M Vinther, SU Andersen, L Krusell, S Thirup, KJ Jensen, CW Ronson, M Blaise, S Radutoiu, J Stougaard. 2015. Receptor-mediated exopolysaccharide perception controls bacterial infection . Nature 523(7560): 308-312. PMID:26153863

AC Muszynski, MA O'Neill, E Ramasamy, S Pattathil, U Avci, MJ Pena, M Libault, MS Hossain, L Brechenmacher, WS York, RM Barbosa, MG Hahn, G Stacey, RW Carlson. 2015. Xyloglucan, galactomannan, glucuronoxylan, and rhamnogalacturonan, I do not have identical structures in soybean root and root hair cell walls. Planta 242(5): 1123-1138. PMID:26067758

D.B. Brown, A. Muszynski, R.W. Carlson. 2013. Elucidation of a novel lipid A alpha-(1,1)-GalA transferase gene (rgtF) from Mesorhizobium loti: heterologous expression of rgtF causes Rhizobium etli to synthesize lipid A with alpha-(1,1)-GalA. Glycobiol 23: 546-558. PMID:23283001

M.R. Davis, A. Muszynski, I.V. Lollett, C.L. Pritchett, R.W. Carlson, J.B. Goldberg. 2013. Identification of the mutation responsible for the temperature-sensitive lipopolysaccharide O-antigen defect in the pseudomonas aeruginosa cystic fibrosis isolate 2192. J. Bacteriol. 195: 1504-1514. PMID:23354750

D.B. Brown, A. Muszynski, O. Salas, K. Speed, R.W. Carlson. 2013. Elucidation of the 3-O-deacylase gene pagL, required for the removal of primary Beta-hydroxyl fatty acid from the lipid A in the nitrogen-fixing endosymbiont Rhizobium etli CE3. J. Biol. Chem. 288: 12004-12013. PMID:23511636

N. Kayagaki, M.T. Wong, I.B. Stowe, S.R. Ramani, L.C. Gonzalez, S. Akashi-Takamura, K. Miyake, J. Zhang, W.P. Lee, A. Muszynski, L.S. Forsberg, R.W. Carlson, V.M. Dixit. 2013. Non-canonical inflammasone activation by intracellular LPS independent of TLR4. Science 341(6151): 1246-1249. PMID:23887873

S. Gao, D. Ren, D. Peng, W.J. Zhang, A. Muszynski, R.W. Carlson, X.X. Gu. 2013. Late acyltransferase genes lpxX and lpsL jointly contribute to the biological activities of Moraxella catarrhalis. J,. Med. Microbiol. 62: 807-812. PMID:23475908

S.J. Kelly, A. Muszynski, Y. Rawaharada, A.M. Hubber, J.T. Sullivan, N. Sandal, R.W. Carlson, J. Stougaard, C.W. Ronson. 2013. Conditional requirement for exopolysaccharide in the Mesorhizobium-Lotus symbiosis. Mol. Plant Microbe. Interact. 26: 319-329. PMID:23134480

A. Muszynski, K. Rabsztyn, K. Knapska, K.A. Duda, K. Duda-Grychtol, K. Kasperkiewicz, J. Radziejewska-Lebrecht, O. Holst, M. Skurnik. 2013. Enterobacterial common antigen and O-specific polysaccharide coexist in the lipopolysaccharide of serotype O:3. Microbiol. Aug:159(Pt8): 1782-1793. PMID:23782803

R.V. Serrato, E. Balsanelli, G.L. Sassaki, R.W. Carlson, A. Muszynski, R.A. Monteiro, F.O. Pedrosa, E.M. Souza, M. Lacomini. 2012. Structural analysis of Herbaspirillum seropedicae lipid-A and of two mutants defective to colonize maize roots.. Int. J. Biol. Bacromol. 51: 384-391. PMID:22676993

A. Muszynski, M.C. Laus, J.W. Kijne, R.W. Carlson. 2011. Structures of the lipopolysaccharides from Rhizobium leguminosarum RBL5523 and its UDP-glucose dehydrogenase mutant (exo5). Glycobiology 21: 55-68. PMID:20817634

A.F. Haag, S. Wehmeier, A. Muszynski, B. Kerscher, V. Fletcher, S.H. Berry, G.L. Hold, R.W. Carlson, G.P. Ferguson. 2011. Biochemical characterization of Sinorhizobium meliloti mutants reveals gene products involved in the biosynthesis of the unusual lipid A very-long-chain fatty acid. J. Biol. Chem. 286: 17455-17456. PMID:21454518

D. Ren, S.W. Yu, S. Gao, D. Peng, T.S. Petralia, A. Muszynski, R.W. Carlson, J.B. Robbins, C.M. Tsai, D.J. Lim, X.-X. Gu. 2011. Mutant lipooligosaccharide-based conjugate vaccine demonstrates a broad-spectrum effectiveneww against Moraxella catarrhalis. Vaccine J 29: 4210-4217. PMID:21501641

M. Biedzka-Sarek, K.J. Clemetson, A. Kateifides, T. Meri, T.S. Jokiranta, A. Muszynski, J. Radziejewska-Lebrecht, V. Zannis, M. Skurnik, M. Jauhiainen. 2011. Apolipoprotein A-I exerts bactericidal activity against Yersinia enterocolitica serotype O:3. J. Biol. Chem. 286(44): 38211-38219. PMID:21896489

R.V. Serrato, G.L. Sassaki, L.M. Cruz, R.W. Carlson, A. Muszynski, R.A. Monteiro, F.O. Pedrosa, E.M. Souza, M. Iacomini. 2010. Chemical composition of lipopolysaccharides isolated from various endophytic nitrogen-fixing bacteria of tyhe genus Herbaspirillum. Can. J. Microbiol. 56: 342-347. PMID:20453901

E.M. Vanderlinde, J.J. Harrison, A. Muszynski, R.W. Carlson, R.J. Turner, C.K. Yost. 2010. Identification of a novel ABC transporter required for desiccation tolerance and biofilm formation in Rhizobium leguminosarum bv. Viciae 3841. FEMS Microbiol. Ecology 71: 327-340. PMID:20030718

S.A. Felek, A. Muszynski, R.W. Carlson, T.M. Tsang, B.J. Hinnebusch, E.S. Krukonia. 2010. Phosphoglucomutase of Yersinia pestis in required for autoaggregation and polymyxin B. resistance. Infect. Immun. 78: 1163-1175. PMID:20028810

S. Soni, R.K. Ernst, A. Muszynski, N.P. Mohapatra, M.B. Perry, E. Vinogradov, R.W. Carlson, J.S. Gunn. 2010. Francisella tularensis blu-gray phase variation involves structural modifications of lipopolysaccharide O-antigen, core and lipid A and affects intramacrophage survival and vaccine efficacy. Frontiers Microbiol. 1: 1-12.

E.M. Vanderlinde, A. Muszynski, J.J. Harrison, S.F. Koval, D.L. Foreman, H. Ceri, E.L. Kannenberg, R.W. Carlson, C.K. Yost. 2009. A Rhizobium leguminosarum biovar viciae 3841 mutant, deficient in 27-hydroxyoctacosanoate-modified lipopolysaccharide is impaired in desiccation tolerance, biofilm formation, and motility. Microbiology 155: 3055-3069. PMID:19460825

S. Gao, D. Peng, W.J. Zhang, A. Muszynski, R.W. Carlson, X.-X. Gu. 2008. Identification of two late acyltransferase genes responsible for lipid AS biosynthesis in Moraxella catarrhalis. FEBS Lett. 275: 5201-5214. PMID:18795947

H.K. Takahashi, R.W. Carlson, A. Muszynski, B. Choudhury, K.S. Kim, D.S. Stephens, H. Watanabe. 2008. Modification of lipooligosaccharide with phosphoethanolamine by LptA in Neisseria meningitides enhances meningococcal adhesion to human endothelial and epithelial cells. Infect. Immun. 76: 5777-5789. PMID:18824535

N.P. Mohapatra, S. Soni, B.L. Bell, R. Warren, R.K. Ernst, A. Muszynski, R.W. Carlson, J.S. Gunn. 2007. Identification of an orphan response regulator required for the virulence of Francisella spp. and transcription of pathogenicity island genes. Infect. Immun. 75: 3305-3314. PMID:17452468

D. Peng, W.-G. Hu, B.P. Choudhury, A. Muszynski, R.W. Carlson, X.-X. Gu. 2007. Role of different moieties from the lipooligosaccharide molecule in biological activities of the Moraxella catarrhalis outer membrane. FEBS J. 274: 5350-5359. PMID:17892485

Book Chapters

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