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Alison Nairn
Associate Research Scientist

Control and regulation of glycan-related gene expression in developmental systems and disease models

Telephone: 706-542-8516
Fax: 706-542-4412

Short Biography
Research Interests

Short Biography:
Dr. Nairn received her B.S. in Microbiology in 1989 and a M.S. in Molecular Biology and Microbiology in 1993 from the University of Central Florida. In 2001, she received her PhD in Biochemistry from the University of Georgia. Her dissertation project involved the cloning and expression of the α-mannosidase from Trypanosoma cruzi under the supervision of Dr. Kelley Moremen. Dr. Nairn worked as a post-doctoral research associate in the Center for Tropical and Emerging Global Diseases for a year to expand her knowledge of Trypanosomes.

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Research Interests:
In 2003, Dr. Nairn joined the CCRC as a postdoctoral research associate to work on transcript analysis of glycan-related genes as part of the NCRR-funded Integrated Technology for Biomedical Glycomics project. During the first several years of the project, methods were developed for analyzing transcripts from mouse tissues and mouse ES cells for >700 glycan-related genes using a qRT-PCR platform. The assembly of a comprehensive mouse gene list and the design of gene-specific primer pairs involved the assistance of an army of energetic undergraduates. The gene list has recently been expanded to include analysis of human cells and tissues.

In order to present the transcript analysis data in a biologically meaningful context, Dr. Nairn used available databases (ie. KEGG) and literature sources to create a series of biochemical pathway diagrams to pair transcript data with the enzyme(s) they encode and the eventual structure(s) that are produced. Through several collaborations with investigators within the CCRC and outside the university, the technology has been applied to transcript analysis in human, mouse, rat, zebrafish, Drosophila and Pine analyses. The eventual goal of the transcript studies is to integrate transcript abundance data with glycan structural analysis developed by other research groups.

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Publications: Author's Last Name: Nairn

Journal Articles
Book Chapters are listed at the bottom of this page.

M.R. Kudelka, A.V. Nairn, M.Y. Sardar, X. Sun, E.L. Chaikof, T. Ju, K.W. Moremen, R.D. Cummings. 2018. Isotopic labeling with cellular O-glycome reporter/amplification (ICORA) for comparative O-glycomics of cultured cells. Glycobiology 28(4): 214-222. PMID:29390058

K.W. Moremen, A. Ramiah, M. Stuart, J. Steel, L. Meng, F. Forouhar, H.A. Moniz, G. Gahlay, Z. Gao, D. Chapla, S. Wang, J.Y. Yang, P.K. Prabhakar, R. Johnson, M.D. Rosa, C. Geisler, A.V. Nairn, J. Seetharaman, S.C. Wu, L. Tong, H.J. Gilbert, J. LaBaer, D.L. Jarvis. 2018. Expression system for structural and functional studies of human glycosylation enzymes. Nature Chem. Biol. 14(2): 156-162. PMID:29251719

CT Saeui, AV Nairn, M Galizzi, C Douville, P Gowda, M Park, V Dharma, S Shah, A Clark, M Austin, KW Moremen, KJ Yarema. 2018. Integration of genetic and metabolic features related to sialic acid metabolism distinguishes human breast cell subtypes. PLoS One 13(5): e0195812-. PMID:29847599

M Kudelka, AV Nairn, MY Sardar, X. Sun, EL Chaikof, KW Moremen, RD Cummings. 2018. Isotopic Labeling with Cellular O-glycome Reporter/Amplification (ICORA) for Comparative O-glycomics of Cultured Cells. Glycobiology 28: 214-222. PMID:29390058

H Qiu, S Shi, J Yue, M Xin, AV Nairn, L Lin, X Liu, G Li, SA Archer-Hartmann, M Dela Rosa, M Galizi, S Wang, F Zhang, P Azadi, TH van Kuppevelt, WV Cardoso, K Kimata, X Ai, KW Moremen, J Esko, RJ Linhardt, L Wang. 2018. A mutant cell library for systematic analysis of heparan sulfate. Nature Meth 15: 889-899. PMID:30377379

S.T. Tuomivaara, P. Schliekelman, A.V. Nairn, K.W. Moremen, W.S. York. 2017. RElative QUantitation Inferred by Evaluating Mixtures (REQUIEM). Anal. Chim. Acta 993: 22-37. PMID:29078952

H Guo, B Zhang, A Nairn, T Nagy, KW Moremen, P Bukhaults, M Pierce. 2017. O-GlcNAc expression levels epigenetically regulate colon cancer tumorigenesis by affecting the cancer stem cell compartment via modulating expression of transcriptional factor MYBL1. J Biol Chem 292: 4123-4137. PMID:28096468

A Deligny, T Dierker, A Dagalv, A Lundequist, I Eriksson, AV Nairn, KW Moremen, CRL Merry, L Kjellen. 2016. NDST2 (N-Deacetylase/N-Sulfotransferase-2) Enzyme Regulates Heparan Sulfate Chain Length. J Biol Chem 291: 18600-18607. PMID:27387504

B McMorran, F McCarthy, E Gibbs, M Pang, J Marshall, A Nairn, KW Moremen, R Crosbie-Watson, L Baum. 2016. Differentiation-related glycan epitopes identify discrete domains of the muscle glycocalyx. Glycobiology 26: 1120-1132. PMID:27236198

R Berger, YH Sun, M Kulik, JK Lee, A Nairn, KW Moremen, M Pierce, S Dalton. 2016. ST8SlA4-dependent polysialylation is a part of a developmental program required for germ layer formation from human pluripotent stem cells. Stem Cells 34: 1742-1752. PMID:27074314

A Magalhaes, R Marcos-Pinto, AV Nairn, M dela Rosa, RM Ferreira, S Junqueira-Neto, D Freitas, J Gomes, P Oliveira, MR Santos, NT Marcos, W Xiaogang, C Figueiredo, C Oliveira, M Dinis-Ribeiro, Carneiro, KW Moremen, L David, CA Reis. 2015. Helicobacter pylori chronic infection and mucosal inflammation switches the human gastric glycosylation pathways. Biochem Biophys Acta 1852: 1928-1939. PMID:26144047

L Gasimli, AM Hickey, B Yang, G Li, M dela Rosa, AV Nairn, MJ Kulik, JS Dordick, KW Moremen, S Dalton, RJ Linhardt. 2014. Changes in glycosaminoglycan structure on differentiation of human embryonic stem cells towards mesoderm and endoderm lineages. Biochim Biophys Acta 1840(6): 1993-2003. PMID:24412195

S Natunen, M Lampinen, H Suila, I Ritamo, V Pitkanen, AV Nairn, J Rabina, S Laitinen, KW Moremen, W Reutter, L Valmu. 2013. Metabolic glycoengineering of mesenchymal stromal cells with N-propanoylmannosamine. Glycobiology 23: 1004-1012. PMID:23708401

L Gasimli, HE Stansfield, AV Nairn, H Liu, JL Paluh, B Yang, JS Dordick, KW Moremen, RJ Linhardt. 2013. Structural remodeling of proteoglycans upon retinoic acid-induced differentiation of NCCIT cells. Glycoconj J 30: 497-510. PMID:23053635

P. Zhao, A.V. Nairn, S. Hester, K.W. Moremen, R.M. ORegan, G. Oprea, L. Wells, M. Pierce, K.L. Abbott. 2012. Proteomic identification of glycosylphosphatidylinositol anchor-dependent membrane proteins elevated in breat carcinoma. J.Biol Chem. 287: 25230-25240. PMID:22654114

D.C. Kraushaar, S. Rai, E. Condac, A.V. Nairn, S. Zhang, Y. Yamaguchi, K. Moremen, S. Dalton, L. Wang. 2012. Heparan sulfate facilitates FGF and BMP signaling to drive mesoderm differentiation of mouse embryonic stem calls. J. Biol. Chem. 287: 22691-22700. PMID:22556407

A.V. Nairn, K. Aoki, M. Dela, M. Porterfield, J.M. Lim, M. Kulik, J.M. Pierce, L. Wells, S. Dalton, M. Tiemeyer, K.W. Moremen. 2012. Regulation of glycan structures in murine embryonic stem cells: Combined transcript profiling of glycan-related genes and glycan structural analysis. J. Biol. Chem. 287: 37835-37856. PMID:22988249

K.W. Moremen, M. Tiemeyer, A.V. Nairn. 2012. Vertebrate protein glycosylation: diversity, synthesis and function. Nat. Rev. Mol. Cell Biol. 13: 448-462. PMID:22722607

H.B. Guo, A. V. Nairn, M. Dela Rosa, T. Nagy, S. Zhao, K. Moremen, M. Pierce. 2012. Transcriptional regulation of the protocadherin beta cluster during Her-2 induced mammary tumorigenesis results from alter N-glycan branching. J. Biol. Chem. 287: 24941-24954. PMID:22665489

A.C. Petrey, H. Flanagan-Steet, S. Johnson, X. Fan, M. Dela Rosa, M.E. Haskins, A.V. Nairn, K.W. Moremen, R. Steet. 2012. Excessive activity of cathepsin K is associated with cartilage defects in a zebrafish model of mucolipidosis II. . Dis Model Mech 5: 177-190. PMID:22046029

H. Park, C.A. Haynes, A.V. Nairn, M. Kulik, S. Dalton, K. Moremen, A.H. Merrill. 2010. Transcript profiling and lipidomic analysis of ceramide subspecies in mouse embryonic stem cells and embryoid bodies. J. Lipid Res. 51: 480-489. PMID:19786568

AV Nairn, M dela Rosa, KW Moremen. 2010. Transcript Analysis in Stem Cells. Meth Enzymol 479: 79-91. PMID:20816160

H.B. Guo, A. Vandersall-Nairn, K. Harris, M. Randolph, G. Alvarez-Manilla, K. Moremen, M. Pierce. 2008. Loss of expression of N-acetylglucosaminyltransferase Va results in altered gene expression of glycosyltransferases and galectins. FEBS Lett. 582: 527-535. PMID:18230362

K.L. Abbott, A.V. Nairn, E.M. Hall, M.B. Horton, J.F. McDonald, K.W. Moremen, D.M. Dinulescu, M. Pierce. 2008. Focused glycomic analysis of the N-linked glycan biosynthetic pathway in ovarian cancer. Proteomics 8: 3210-3220. PMID:18690643

A.V. Nairn, W.S. York, K. Harris, E.M. Hall, J.M. Pierce, K.W. Moremen. 2008. Regulation of glycan structures in animal tissues: transcript profiling of glycan-related genes. J. Biol. Chem. 283: 17298-17313. PMID:18411279

A.V. Nairn, A. Kinoshita-Toyoda, H. Toyoda, J. Xie, K. Harris, S. Dalton, M. Kulik, J.M. Pierce, T. Toida, K.W. Moremen, R.J. Linhardt. 2007. Glycomics of proteoglycan biosynthesis in murine embryonic stem cell differentiation. J. Proteome Res. 6: 4374-4387. PMID:17915907

R. Zhong, M.J. Peña, G-K. Zhou, C.J. Nairn, A. Wood-Jones, E.A. Richardson, W.H. Morrison, A.G. Darvill, W.S. York, Z-H. Ye. 2005. Arabidopsis Fragile Fiber8, which encodes a putative glucuronyltransferase, is essential for normal seccondary wall synthesis. Plant Cell 17: 3390-3408. PMID:16272433

D.S. Gonzalez, K. Karaveg, A.S. Vandersall-Nairn, A. Lal, K.W. Moremen. 1999. Identification, expression, and characterization of a cDNA encoding human endoplasmic reticulum mannosidase I, the enzyme that catalyzes the first mannose trimming step in mammalian Asn-linked oligosaccharide biosynthesis. J. Biol. Chem. 274: 21375-21386. PMID:10409699

A. Vandersall-Nairn, K. O'Brien, R.K. Merkle, T.N. Oeltmann, K.W. Moremen. 1998. Cloning, expression, purification, and characterization of the acid alpha-mannosidase from Trypanosoma cruzi. Glycobiology 8: 1183-1194. PMID:9858640

Book Chapters

AV Nairn, KW Moremen. 2015. Glycotranscriptomics in Glycoscience: Biology and Medicine (N. Taniguchi, T. Endo, G. Hart, P. Seeberger, C.-H. Wang). In: , pp. 1475-1482. Springer-Verlag, Tokyo.

A.V. Nairn, K.W. Moremen. 2014. Glucosidase, alpha neutral AB; glucosidase II subunit beta (GANAB, PRKCSH, alpha-glucosidase II). In: Handbook of Glycosyltransferases and Related Genes, 2nd edition (Taniguchi N. ed), pp. 1283-1295. Springer-Verlag, Tokyo.

A.V. Nairn, K.W. Moremen. 2014. Mannosyl-oligosaccharide glucosidase (Glucosidase I, MOGS). In: Handbook of Glycosyltransferases and Related Genes, Vol.2nd edition:1273-1282. Springer-Verlag, Tokyo.

K.W. Moremen, A.V. Nairn. 2014. Mannosidase, alpha, class 2a1 (MAN2A1, Golgi alpha-mannosidase II). In: Handbook of Glycosyltransferases and Related Genes, 2nd edition, pp. 1313-1326. Springer-Verlag, Tokyo.

K.W. Moremen, A.V. Nairn. 2014. Mannosidase, alpha, class 1 (MAN1A1) (Golgi alpha-, class 1 (MAN1A1 (Golgi alpha-mannosidase 1A), Man1A2 (Golgi alpha-mannosidase 1B), MAN1B1 (ER alpha-mannosidase I), MAN1C1 (Golgi alpha-mannosidase 1C). In: Handbook of Glycosyltransferases and Related Genes, 2nd edition, pp. 1297-1312. Springer-Verlag, Tokyo.

A. Vandersall-Nairn, K. Moremen. 2009. Glycotranscriptomics. In: Handbook of Glycomics (R.D. Cummings and J.M. Pierce, eds.), pp. 81-136. Academic Press.

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