The Biomolecular Interaction Laboratory at the CCRC provides campus researchers surface plasmon
resonance (SPR) for real-time monitoring of macromolecular interactions without the need for labels via
the Biacore optical biosensor.
Dr. Parastoo Azadi is the coordinator of this laboratory, which currently has a Biacore 3000
and a Biacore T100 utilizing BIAcontrol software. Biacore instruments are available on collaboration or
a fee-for-service basis to researchers at the University of Georgia as well as researchers from other institutions or companies.
SPR systems monitor molecular interactions in real-time using a label-free detection method. A sample
in solution is injected over a sensor surface on which the potential interacting partner(s) are
immobilized, either singly in individual flow cells or as part of an array. As the injected sample
interacts with the immobilized partners, the refractive index at the interface between the sensor surface
and the solution alters to a degree proportional to the change in mass at the surface. The phenomenon of surface plasmon
resonance (SPR) is exploited to detect these changes in real time and data are presented in a 'sensogram', a profile of
SPR response plotted against time. The sensogram traces the association and dissociation of complexes over
the entire course of an interaction, with the kinetics revealed by the shape of the binding curve.
The Biacore 3000 is our basic tool for exploring protein function and is useful for samples ranging
from small molecules to crude extracts, lipid vesicles, viruses, bacteria and eucaryotic cells. The
Biacore 3000 is designed for individual sample characterization.
The Biacore T100 can do everything that the 3000 can do, plus analyse low MW drug candidates to high
molecular weight proteins (also DNA, RNA, polysaccharides, lipids, cells and viruses), in various samples
environments, e.g. In DMSO-containing buffers, plasma and serum. The injection volume range from 2-350 μL
(application dependent) and the range of flow rate are from 1-100 μL/min. The disadvantage of the T-100
is that it is less tolerant of impurities as compared to the 3000. Also, the cost of supplies is somewhat
Typical working ranges:
* Association rate constant (ka): 103-107
M-1s-1 (and higher for macromolecular analytes)
* Dissociation rate constant (kd): 10-5-0.5 s-1
* Sample concentration: 10-3-10-11M
* Molecular weight detection: >100 Da
* Recovery specifications: 1.5 μL analyte recovery volume
Policies and Procedures
All new users need to be trained or provide convincing evidence of past training. Each user will
be responsible for keeping track of sensor chips, supplies, reagents, and disposables used; clean-up of
the immediate laboratory area; and specified maintenance of the instrument.
Investigators must purchase a Biacore Getting Starter Kit (BR-1005-50 for Biacore 300 and BR-1006-50
for Biacore T100) and a CM-5 chip (catalogue # BR-1000-12 for Biacore 3000 and catalogue # BR-1005-30 for Biacore T100) that will be used during training. After
training is completed the investigator can schedule time on the instrument.
Sign up is done in one-day blocks and you are allowed to sign for a maximum of 3 days in a row,
including the weekend. Please contact Parastoo Azadi at firstname.lastname@example.org
(706-583-0629) or visit The Complex Carbohydrate Research Center, Office 1032.
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For more information please contact:
Parastoo Azadi, Ph.D.
Executive Technical Director - Analytical Services
Complex Carbohydrate Research Center
The University of Georgia
315 Riverbend Road
Athens, Georgia, 30602, USA