Transcript analysis of glycan related genes by real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR).

We have developed a high-throughput methodology for transcript analysis of glycan related genes (transcripts from >700 genes) by a qRT-PCR approach. The power of the technique is its extreme sensitivity and the wide dynamic range for quantitation of both high and low i abundance transcripts. The focus of our studies will be to assess transcript abundance by qRT-PCR for genes encoding all of the "glycan-related" glycosyltransferases, glycosidases, transporters, modification enymes, sudar-nucleotide biosynthetic enzymes and lectins (>700 genes). Interested parties should feel free to contact Dr. Kelley Moremen ( or Dr. Parastoo Azadi ( ).