Professor of Biochemistry and Molecular Biology
Biosynthesis and biological functions of pectin
keywords and Selected Recent Publications
Lab-personal web site
Debra MohnenProfessor of Biochemistry and Molecular Biology and
Adjunct Professor in Plant Biology
Biosynthesis, structure and function of plant cell wall polysaccharides and glycoconjugates with emphasis on pectin biosynthesis and
structure-function relationships in the wall.
Dr. Debra Mohnen received her B.A. in biology from Lawrence University (Wisconsin) and her M.S. in botany and Ph.D. in plant biology from the
University of Illinois with Ph.D. research conducted largely at the Friedrich Miescher Institute in Basel, Switzerland.
She held postdoctoral research associate positions at the USDA's Richard Russell Research Center and at the Complex Carbohydrate Research Center (CCRC)
in Athens, GA where she won an NIH National Research Service Award for her research. She was appointed to the CCRC faculty in September 1990 and is
currently Professor in the Department of Biochemistry and Molecular Biology, adjunct faculty member in the Department of Plant Biology, and member
of the Plant Center at UGA. Dr. Mohnen is Focus Area Lead for Plant Biomass Formation and Modification in the DOE-funded BioEnergy Science Center (BESC).
She has served on the Committee on the Status of Women in Plant Physiology of the American Society of Plant Physiologists, invited faculty sponsor for
the UGA Association for Women in Science (AWIS), past member-at-large in the Cellulose and Renewable Materials Division of the American Chemical Society,
and past member of the Council for Chemical and Biochemical Sciences, Chemical Sciences, Geosciences, and Biosciences Division in the Office of
Basic Energy Sciences, Office of Science, U.S. Department of Energy. As Co-PI on the NSF-funded "Plant Cell Wall Biosynthesis Research Network" Dr. Mohnen
established and continues to direct "CarboSource Services" which provides rare substrates for plant wall polysaccharide synthesis to the research community.
Dr. Mohnen was awarded the 2008 Bruce Stone Award by the Plant Polysaccharide Workshop for contributions and promising research in the area of Pectin
Biosynthesis. She received the Student Government Association Certificate of Recognition for Outstanding Commitment to the Students of the University
of Georgia in 2008 and the Outstanding Faculty Research Mentor award from Division of Biological Sciences, Franklin College of Arts and Sciences,
University of Georgia in 2012. Dr. Mohnen was Chair of the 2009 Plant Cell Walls Gordon Research Conference, serves as an Editor-in-Chief for
Biotechnology for Biofuels, and serves on scientific advisory boards for multiple bioenergy centers. She was elected as an AAAS Fellow in 2013.
Dr. Mohnen’s research focuses on the biosynthesis, function and structure of plant cell wall polysaccharides and glycoconjugates, with emphasis on pectin, matrix polysaccharides and wall proteoglycans.
The research goals include:
*Understanding the structure, biosynthesis and function of wall polymers that contain pectic glycans.
*Improving plant growth and development, and the use and conversion of plant cell wall biomass to biofuels and bioproducts, through modification of wall structure and synthesis.
*Reevaluation of plant cell wall models based on recent data that are inconsistent with recently identified wall matrix polymer structures.
The research includes biochemical, chemical, molecular genetic and genomic methods and use of both model systems (e.g. Arabidpsis and rice)
and biomass feedstock (e.g. Populus and switchgrass)
Keywords and Selected Recent Publications:Keywords: Pectin biosynthesis, hemicellulose biosynthesis, oligogalacturonides, plant biochemistry and molecular biology, plant morphogenesis, organogenesis, plant cell walls, homogalacturonan, RG-I, RG-II, galacturonosyltransferase, glycosyl transferase, GAUT1-related gene family, cotton fiber, apoptosis, cancer, biofuel, bioenergy
Selected Recent Publications
Mohnen, D. (2008) Pectin Structure and Synthesis. Curr. Opin. Plant Biology 11:266-277.
Etzler, M.E. and Mohnen, D. (2008) Green Plants. In Essentials of Glycobiology, Second Edition, eds. Varki, A., Cummings, R., Esko, J., Freeze, H., Stanley, P., Bertozzi, C., Hart, G. and Etzler, M. Cold Spring Harbor Laboratory Press, Chapter 22.
Mohnen, D., Bar-Peled, M. and Somerville, C. (2008) Biosynthesis of Plant Cell Walls. In Biomass Recalcitrance, ed. Himmel, M., Blackwell Publishing, Oxford. Chapter 5, pgs. 94-187.
Jackson, C.L., Dreaden, T.M., Theobald, L.K., Tran, N.M.; Beal, T.L., Eid, M., Gao, M.Y., Shirley, R.B., Stoffel, M.T., Kumar, M.V. Mohnen, D. 2007 Pectin induces apoptosis in human prostate cancer cells: correlation of apoptotic function with pectin structure. Glycobiology 17:805-819.
Persson, S., Caffall, K.H., Freshour, G. Hilley, M.T., Bauer, S., Poindexter, P., Hahn, M., Mohnen, D., Somerville, C. 2007. The Arabidopsis irregular xylem 8 mutant is deficient in glucuronoxylan and homogalacturonan which are essential for secondary cell wall integrity. Plant Cell 19:237-255.
Sterling, J.D., M.A. Atmodjo, S.E. Inwood, V.S.K. Kolli, H.F. Quigley, M.G. Hahn, and D. Mohnen. 2006. Functional identification of an Arabidopsis pectin biosynthetic homogalacturonan galacturonosyltransferase. Proc. Natl. Acad. Sci. 103: 5236-5241.
Sterling, J.D., J.A. Lemons, I.F. Forkner, and D. Mohnen. 2005. Development of a filter assay for measuring homogalacturonan:a-1,4-galacturonosyltransferase activity. Anal. Biochem. 343: 231-236.
Guillaumie, F., J.D. Sterling, K.J. Jensen, O.R.T. Thomas, and D. Mohnen. 2003. Solid-supported enzymatic synthesis of pectic oligogalacturonides and their analysis by MALDI-TOF mass spectrometry. Carbohydr. Res. 338: 1951-1960.
Mohnen, D. 2002. Biosynthesis of pectins. In: GB Seymour, JP Knox, eds Pectins and their Manipulation. Blackwell Publishing and CRC Press, Oxford, pp 52-98.
Sterling, J., H.F. Quigley, A. Orellana, and D. Mohnen. 2001. The catalytic site of the pectin biosynthetic enzyme a-1,4-galacturonosyltransferase (GalAT) is located in the lumen of the Golgi. Plant Physiol. 127: 360-371.
Ridley, B.L., M.A. O'Neill, and D. Mohnen. 2001. Pectins: structure, biosynthesis, and oligogalacturonide-related signaling. Phytochemistry 57: 929-967.
Goubet, F. and D. Mohnen. 1999. Subcellular localization and topology of homogalacturonan methyltransferase in suspension-cultured Nicotiano tabacum cells. Planta 209: 112-117.
Goubet, F. and D. Mohnen. 1999. Solubilization and partial characterization of homogalacturonan-methyltransferase from microsomal membranes of suspension-cultured tobacco cells. Plant Physiol. 121: 281-290.
Orellana, A. and D. Mohnen. 1999. Enzymatic synthesis and purification of [3H] uridine diphosphate galacturonic acid for use in studying Golgi-localized transporters. Anal. Biochem. 272: 224-231.
Scheller, H.V., R.L. Doong, B.L. Ridley, and D. Mohnen. 1998. Pectin biosynthesis: a solubilized a1,4-galacturonosyltransferase from tobacco catalyzes the transfer of galacturonic acid from UDP-galacturonic acid onto the non-reducing end of homogalacturonan. Planta 207: 512-517.
Doong, R.L. and D. Mohnen. 1998. Solubilization and characterization of a galacturonosyltransferase that synthesizes the pectic polysaccharide homogalacturonan. Plant J. 13: 363-374.
Goubet, F., L.N. Council, and D. Mohnen. 1998. Identification and partial characterization of the pectin methyltransferase "homogalacturonan-methyltransferase" from membranes of tobacco cell suspensions. Plant Physiol. 116: 337-347.
Ridley, B.L., M.D. Spiro, J. Glushka, P. Albersheim, A. Darvill, and D. Mohnen. 1997. A method for biotin labeling of biologically active oligogalacturonides using a chemically stable hydrazide linkage. Anal. Biochem. 249: 10-19.
Doong, R.L., K. Liljebjelke, G. Fralish, A. Kumar, and D. Mohnen. 1995. Cell free synthesis of pectin: identification and partial characterization of polygalacturonate 4-a-galacturonosyltransferase and its products from membrane extracts of tobacco cell suspension cultures. Plant Physiol. 109: 141-152.
Liljebjelke, K., R. Adolphson, K. Baker, R.L. Doong, and D. Mohnen. 1995. Enzymatic synthesis and purification of uridine diphosphate [14C]galacturonic acid: a substrate for pectin biosynthesis. Anal. Biochem. 225: 296-304.