Georgia Research Alliance Lars G. Ljungdahl Distinguished Investigator, Director of Graduate Studies & Professor Biochem. and Mol. Biol., Adjunct Prof. Chem., Georgia Cancer Coalition Scholar
Roles of regulatory post-translational modifications, in particular O-glycosylation, in increasing functional diversity of proteins.
keywords and Selected Recent Publications
Short Biography:Dr. Wells received his B.S. in Chemistry, with a minor in Psychology, in 1991 from the Georgia Institute of Technology, and after spending two years working at the Microchemical Facility, his Ph.D. in Biochemistry and Molecular Biology in 1998 from the Emory University School of Medicine. A postdoctoral research fellowship at the Johns Hopkins School of Medicine in Biological Chemistry followed, which was supported by a National Research Service Award from the National Cancer Institute of the NIH. Dr. Wells joined the CCRC in August of 2003. Full publications: 98.
Using a combination of methodologies, including mass spectrometry, protein biochemistry, cell biology, genetics, proteomics, and molecular biology, we study the role of PTMs (primarily O-glycosylation) in a variety of pathophysiological processes including cancer, diabetes, viral infection, neurological disorders, and congenital muscular dystrophy. Our research is aimed at increasing our understanding of how increased functional diversity leads to finer control of biological processes. The hope is that by understanding the role of PTMs, we will not only more accurately describe fundamental biological processes but will also elucidate novel therapeutic targets in disease states where these processes have become dysregulated.
Keywords and Selected Recent Publications:
Keywords: O-GlcNAc, O-Man, glycosylation, phosphorylation, signal transduction, post-translational modification, mass spectrometry, proteomics, diabetes, cancer, congenital muscular dystrophy
Selected Recent Publications:
Yoshida-Moriguchi T, Davis S, Hammond S, Yu L, Kunz S, Madson M, Oldstone MBA, Schachter H, Wells L, Campbell KP (2009) O-mannosyl phosphorylation of alpha-dystroglycan: A novel modification required for laminin-binding. Science. In press.
Humbard MA, Miranda HV, Lim J, Krause DJ, Pritz JR, Zhou G, Chen S, Wells L, Maupin-Farlow JA (2009) Ubiquitin-like Small Archaeal Modifier Proteins (SAMPs) in Haloferax volcanii. Nature. In press.
Hale CR, Zhao P, Olson S, Duff MO, Gravely BR, Wells L, Terns RM, Terns MP (2009) RNA-guided RNA cleavage by a CRISP RNA-Cas protein complex. Cell. In press.
Orlando R, Lim J, Atwood J, Angel P, Fang M, Aoki K, Alvarez-Manilla G, Moremen K, York W, Tiemeyer M, Pierce M, Dalton S, Wells L (2009) IDAWG: Metabolic incorporation of stable isotope labels for quantitative glycomics of cultured cells. J. Proteome Res. 8: 3816-3823.
Webster DM, Teo CF, Sun Y, Wloga D, Gay S, Klonowski KD, Wells L, Dougan ST (2009) O-GlcNAc modifications regulate cell survival and epiboly during zebrafish development. BMC Develop. Biol. 9: 28.
Li Y, Cao C, Jia W, Yu L, Mo M, Wang Q, Huang Y, Lim JM, Ishihara M, Wells L, Azadi P, Robinson H, He YW, Zhang L, Mariuzza RA (2009) Structure of the F-spondin domain of mindin, an integrin ligand and pattern recognition molecule. EMBO J. 28: 286-297.
Lim JM, Sherling D, Teo CF, Hausman DB, Lin D, Wells L (2008) Defining the regulated secreted proteome of rodent adipocytes upon the induction of insulin resistance. J. Proteome Res. 7: 1251-1263.